Urinary Concentrations of Four Parabens in the U.S. Population: NHANES 2005–2006
Antonia M. Calafat, Xiaoyun Ye, Lee-Yang Wong, Amber M. Bishop, Larry L. Needham
Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
Environ Health Perspect 118:679-685 (2010). http://dx.doi.org/10.1289/ehp.0901560 [online 04 January 2010]
Background: Parabens are widely used as antimicrobial preservatives in cosmetics, -pharmaceuticals, and food and beverage processing.
Objectives: We assessed exposure to methyl, ethyl, propyl, and butyl parabens in a representative sample of persons ≥ 6 years of age in the U.S. general population from the 2005–2006 National Health and Nutrition Examination Survey.
Methods: We analyzed 2,548 urine samples by using online solid-phase extraction coupled to isotope dilution–high-performance liquid chromatography/tandem mass spectrometry.
Results: We detected methyl paraben (MP) and propyl paraben (PP) in 99.1% and 92.7% of the samples, respectively. We detected ethyl (42.4%) and butyl (47%) parabens less frequently and at median concentrations at least one order of magnitude lower than MP (63.5 µg/L) and PP (8.7 µg/L). Least-square geometric mean (LSGM) concentrations of MP were significantly higher (p ≤ 0.01) among non-Hispanic blacks than among non-Hispanic whites except at older ages (≥ 60 years). Adolescent and adult females had significantly higher (p < 0.01) LSGM concentrations of MP and PP than did adolescent and adult males. Females were more likely than males [adjusted odds ratios (ORs) and 95% confidence intervals (CIs): MP, 3.2 (2.99–5.27); PP, 4.19 (2.34–7.49)] and non-Hispanic blacks were more likely than non-Hispanic whites [MP, 4.99 (2.62–9.50); PP, 3.6 (1.86–7.05)] to have concentrations above the 95th percentile.
Conclusions: The general U.S. population was exposed to several parabens during 2005–2006. Differences in the urinary concentrations of MP and PP by sex and race/ethnicity likely reflect the use of personal care products containing these compounds.
Key words: biomonitoring, exposure, human, NHANES, p-hydroxybenzoic acid esters, urine
Address correspondence to A.M. Calafat, Centers for Disease Control and Prevention, National Center for Environmental Health, Division of Laboratory Sciences, 4770 Buford Hwy., NE, Mailstop F53, Atlanta, GA 30341 USA. Telephone: (770) 488-7891. Fax: (770) 488-4609. E-mail: Acalafat@cdc.gov
The authors declare they have no actual or potential competing -financial interests.
We thank J. Reidy and L.T. Jia for technical assistance.
Received 09 October 2009; accepted 04 January 2010; online 04 January 2010.
The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the Centers for Disease Control and Prevention.
Parabens are esters of p-hydroxybenzoic acid. They are used as antimicrobial preservatives, especially against molds and yeast, in cosmetics, pharmaceuticals, and food and beverage processing (Andersen 2008; Elder 1984). Parabens are popular preservatives because of their low toxicity and cost, their broad inertness, and their worldwide regulatory acceptance (Soni et al. 2005). Methyl paraben (MP) and propyl paraben (PP) are two of the most used parabens (Andersen 2008).
Parabens are not mutagenic (Elder 1984), but their potential estrogenic activity—which is orders of magnitude lower than that of estrogen (Andersen 2008; Golden et al. 2005)—has raised some concerns. The presence of parabens in human breast tumors (Darbre et al. 2004) triggered a debate about their use in cosmetics, particularly in underarm deodorants and antiperspirants, and the incidence of breast cancer (Darbre 2006; Darbre and Harvey 2008; Golden et al. 2005; Harvey and Darbre 2004). However, the Scientific Committee on Consumer Products (SCCP) of the European Commission concluded that there was no evidence that using paraben-containing underarm cosmetics increased the demonstrable risk of breast cancer (SCCP 2005). In 2003, the Cosmetic Ingredient Review (CIR) expert panel, part of a program established by the Cosmetics, Toiletry, and Fragrance Association, with support from the U.S. Food and Drug Administration and the Consumer Federation of America, decided to reevaluate the safety of parabens (Bergfeld et al. 2005) and to assess their link to endocrine disruption. In 2006, the CIR panel concluded that methyl, ethyl, propyl, isopropyl, butyl, isobutyl, and benzyl parabens are safe as cosmetic ingredients in the practices of use and use concentrations (up to 0.4% if used alone, up to 0.8% in mixtures) (Andersen 2008). Nonetheless, the National Institute of Environmental Health Sciences has recommended butyl paraben (BP) for toxicologic evaluation because of its widespread use, its potential reproductive toxicity, and the lack of adequate toxicologic data (National Toxicology Program 2004, 2006). Furthermore, SCCP recently concluded that the safety assessment of PP and BP could not yet be finalized on the basis of available data (SCCP 2008).
Exposure to parabens may occur through inhalation, dermal contact, and ingestion (El Hussein et al. 2007; Soni et al. 2005), and metabolism may differ, depending upon the exposure route (Bando et al. 1997; Soni et al. 2005). Also, metabolism and uptake from human skin may be lower than in other species (Harville et al. 2007; Jewell et al. 2007a; Prusakiewicz et al. 2006), and interindividual differences among humans in dermal metabolic capacity exist (Jewell et al. 2007b). Parabens can be hydrolyzed to p-hydroxybenzoic acid, which can be conjugated before urinary excretion (Kiwada et al. 1979, 1980), but they can also be excreted as intact esters (Ye et al. 2006a). The fraction of the parabens excreted in the urine as the parent paraben (free or conjugated) versus the fraction excreted as p-hydroxybenzoic acid is unknown. In humans, p-hydroxybenzoic acid, a nonspecific metabolite of all parabens, and its conjugates in urine are not optimal biomarkers of exposure to parabens, known to have quite different bioactivities. By contrast, the concentrations of total (free plus conjugated) urinary species of the parent parabens can be used as valid human exposure bio-markers (Ye et al. 2006a).
Our evaluation of the urinary concentrations of parabens among 100 adults with no known occupational exposure to the compounds showed widespread exposure to these chemicals (Ye et al. 2006a). Here we report the first nationally representative data on the total concentrations of four parabens in urine among those in the U.S. general population ≥ 6 years of age, stratified by age group, sex, and race/ethnicity.
Since 1999, the Centers for Disease Control and Prevention (CDC) has conducted annually the National Health and Nutrition Examination Survey (NHANES) to measure the health and nutritional status of the civilian noninstitutionalized U.S. population ≥ 2 months of age (CDC 2009b). NHANES includes household interviews, standardized physical examinations, and collection of medical histories and biologic specimens. Some of these specimens are used to assess exposure to environmental chemicals (CDC 2009b). For this study, 2,548 urine specimens were collected from a one-third subset of participants ≥ 6 years of age. The representative design of the survey was maintained because the subset was random. The National Centers for Health Statistics Institutional Review Board reviewed and approved the study protocol. All participants gave informed written consent; parents or guardians provided consent for participants < 18 years of age.
Participants provided one spot urine sample during one of three daily examination sessions. The samples were shipped on dry ice to CDC’s National Center for Environmental Health and stored at temperatures below –20°C until analyzed. We measured the concentrations of total parabens, described in detail elsewhere (Ye et al. 2006b). We first hydrolyzed the conjugated species of parabens in 100 µL urine by use of β-glucuronidase/sulfatase (Helix pomatia, H1; Sigma Aldrich Laboratories, Inc., St. Louis, MO). The parabens were preconcentrated by online solid-phase extraction, separated from other urine components by reversed-phase high-performance liquid chromatography, and detected by atmospheric pressure chemical ionization–isotope dilution/tandem mass spectrometry with peak focusing (Ye et al. 2006b). The limits of detection (LODs) in synthetic urine, calculated as 3S0, where S0 is the standard deviation as the concentration approaches zero (Taylor 1987), were 1.0 µg/L [MP, ethyl paraben (EP)] and 0.2 µg/L (PP, BP). We prepared low-concentration (2.2–9.0 µg/L) and high-concentration (10.5–53.8 µg/L) quality control materials (QCLs and QCHs, respectively) with pooled human urine that was analyzed with standards, reagent blanks, and NHANES samples. The precision of measurements, expressed as the relative standard deviation of 55–66 meas-ures, depending on the analyte, was 5.8–12.1% for QCLs and 4.4–5.6% for QCHs.
We used SAS (version 9.1.3; SAS Institute Inc., Cary, NC) and SUDAAN (version 10; Research Triangle Institute, Research Triangle Park, NC) to perform statistical analyses. SUDAAN calculates variance estimates after incorporating the sample population weights, used to produce estimates that are representative of the U.S. population, which account for nonresponse rates, unequal selection probabilities, and planned oversampling of certain subgroups resulting from the complex multistage probability design of NHANES. We stratified age, reported in years at the last birthday, in four groups (6–11, 12–19, 20–59, and ≥ 60 years). On the basis of self-reported data, we categorized race/ethnicity as non-Hispanic black, non-Hispanic white, and Mexican American. Participants not defined by these racial/ethnic categories were included only in the total population estimate. For each age, sex, and race/ethnic group, we calculated the geometric means (if the overall weighted frequency of detection was > 60%) and distribution percentiles for both volume-based (micrograms per liter) and creatinine-corrected (micrograms per gram creatinine) concentrations. For concentrations below the LOD, we used a value equal to the LOD divided by the square root of 2 in univariate and multivariate analyses, as recommended for examining NHANES data (CDC 2009a).
We used analysis of covariance to examine whether several variables, selected on the basis of statistical, demographic, and biological considerations, were associated with the log-transformed urine concentrations of MP and PP. We considered age (continuous), sex, race/ethnicity, creatinine concentration, household income (described below), and examination session (i.e., morning, afternoon, evening). On the basis of questionnaire responses, annual household income was available in increments of $5,000 (ranging from < $5,000 to > $75,000). We categorized income as < $20,000, $20,000–$45,000, and > $45,000 to obtain a comparable number of participants per group. For the multiple regression models, we used the variables described previously and all their possible two-way interactions to calculate the adjusted least-square geometric mean (LSGM) concentrations (micrograms per liter) of MP and PP. These variables were log transformed because the distributions of concentrations of these parabens and creatinine were skewed.
To arrive at the final model, we used backward elimination with SUDAAN to remove the nonsignificant interactions one at a time for each analyte. Nonsignificant main effects were then removed one at a time, and the model was rerun to determine whether the β-coefficients for significant main effects or interactions changed by > 10%. If any did, we retained the nonsignificant main effect in the model. Once the backward procedure was completed, main effects and interactions were added back into the model one at a time to determine whether any were significant (p < 0.05). We retained those that were in the final model.
We also conducted weighted univariate and multiple logistic regressions to examine the association of MP and PP concentrations above the 95th percentile with sex, age group, race/ethnicity, household income, and examination session.
We detected MP at concentrations ranging from 1.0 to 17,300 µg/L in 99.1% of persons examined. We detected PP in 92.7% of persons at concentrations of 0.2–7,210 µg/L. We detected EP and BP less frequently and at lower concentration ranges (EP: 42.4%, 1.0–1,110 µg/L; BP: 47%, 0.2–1,240 µg/L) than MP and PP. The geometric mean and 95th percentile concentrations were 56.4 µg/L (55.0 µg/g creatinine) and 974 µg/L (902 µg/g creatinine) for MP, and 7.72 µg/L (7.53 µg/g creatinine) and 299 µg/L (263 µg/g creatinine) for PP, respectively (Tables 1 and 2). Because the weighted frequency of detection was < 60% for BP (except for females) and EP, we did not calculate the geometric mean (Tables 3 and 4) or conduct multivariate analysis.
We found that the concentrations of MP and PP were highly correlated [Pearson correlation coefficient (R) = 0.62350, p < 0.0001]. We also observed significant (p < 0.0001), but not as strong (R = 0.49637), correlations between the concentrations of BP and EP. The correlations between MP or PP and both BP and EP were weaker (R = 0.17911–0.34222) yet statistically significant (p < 0.0001).
The final MP and PP models included log creatinine, income, and the interaction terms sex × race/ethnicity (only for PP), sex × age (p < 0.01), and age × race/ethnicity (p < 0.01) (Table 5). For both parabens, LSGM concentrations for persons in the high household income category were significantly higher than for those in the low (p < 0.01) and medium (MP, p = 0.05; PP, p < 0.01) categories (Table 5). Females had significantly higher concentrations (p < 0.01) of both parabens than did males for all age groups, except children (MP, p = 0.11; PP, p = 0.81) (Figure 1); females also had significantly higher PP concentrations (p < 0.01) than did males, regardless of race/ethnicity (Table 5). Regardless of sex, adults ≥ 60 years of age had significantly higher LSGM concentrations of both parabens than did children (MP, p = 0.05; PP, p < 0.01) and adolescents (p = 0.03). Children had significantly lower LSGM concentrations than did adults 20–59 years of age (MP, p = 0.03; PP, p < 0.01) and adolescents (p = 0.04 for PP only) (Table 5, Figure 1). Except for persons ≥ 60 years of age, LSGM concentrations of MP were significantly higher for non-Hispanic blacks than for non-Hispanic whites (p < 0.01; Table 5, Figure 2). Similarly, non-Hispanic black children and adolescents (p < 0.01) had significantly higher concentrations of MP than Mexican Americans. We found the same pattern for PP (Table 5), except that the differences for adolescents were only of borderline significance (p = 0.07). Mexican Americans had higher LSGM concentrations of both MP and PP than did non-Hispanic whites (Table 5), but the differences reached statistical significance (p < 0.01) only among adults and adolescents for MP. Among Mexican Americans, adults had significantly higher LSGM concentrations (p < 0.01) of both MP and PP than did children and adolescents; the LSGM concentrations among children were significantly lower than those for adults ≥ 60 years of age (p < 0.01) and adolescents (MP, p = 0.01; PP, p < 0.01). Among non-Hispanic whites, adults and older adults had significantly higher LSGM concentrations than did adolescents (MP, p < 0.01; PP, p = 0.03 for adults and p < 0.01 for older adults) and children (p < 0.01) (Table 5). Among non-Hispanic blacks, adults had significantly higher LSGM concentrations of MP and PP than did children (MP, p = 0.02; PP, p < 0.01), and adolescents also had significantly higher LSGM concentrations of PP than did children (p = 0.01) (Table 5).
For both MP and PP, the likelihood of having a urinary concentration above the 95th percentile (an arbitrary value selected as an example of higher than average concentrations) was significantly associated with sex (p < 0.01) and race (p < 0.01) but not with age, income, or examination session. Females were about three to four times more likely than males to have urinary concentrations of MP and PP above the 95th percentile [adjusted odds ratios (ORs) with 95% confidence intervals (CIs): MP, 3.2 (2.99–5.27); PP, 4.19 (2.34–7.49)]. Compared with non-Hispanic whites, the likelihood of having urinary concentrations of MP or PP at or above the 95th percentile was two to five times higher for non-Hispanic blacks [MP, 4.99 (2.62–9.50); PP, 3.6 (1.86–7.05)] and for Mexican Americans (MP, 2.03 (1.30–3.17); PP, 2.56 (1.56–4.24)]. Non-Hispanic blacks were about 2.5 times more likely than Mexican Americans to have MP concentrations above the 95th percentile [2.45 (1.41–4.28)], but we found no significant difference (p = 0.13) between non-Hispanic blacks and Mexican Americans for PP.
We detected concentrations of free plus conjugated species of MP and PP in urine in > 92% of the samples examined; we detected EP and BP in about 50%. The high frequency of detection of MP and PP most likely resulted from their wide use in food products (Soni et al. 2005) and in common personal care products (e.g., lotions, cosmetics, hair preparations) (Andersen 2008). The range of urinary concentrations spanning up to three orders of magnitude (Tables 1–4) may be related to lifestyle factors, including diet, that result in exposure differences and/or to individual variations in bioavailability, distribution kinetics, or metabolism of the parabens. These factors are important in interpreting biomonitoring data for other chemicals found in personal care products, such as the bactericide triclosan (Calafat et al. 2008b; Sandborgh-Englund et al. 2006) and the sunscreen agent benzophenone-3 (Calafat et al. 2008a). All these factors as well as the timing of sample collection will affect the urinary concentrations of parabens on an individual basis. On a population basis (e.g., NHANES), however, the wide range of concentrations observed would represent an average exposure scenario (i.e., a paraben urinary concentration in the upper percentiles resulting from the collection of the urine soon after a person’s paraben-related activity may be offset by a concentration in the lower percentiles of another person who provided a urine sample shortly before conducting the same activity).
We observed moderate correlations between the concentrations of EP and BP, and much weaker correlations between either of these parabens and MP or PP. These findings suggest a potential common source(s) of exposure for EP and BP, which was not the case for MP and PP. The concentrations of MP and PP were highly correlated, most likely because they are the two most common parabens (Soni et al. 2005) and may be used in combination in many commercial applications, including food, pharmaceuticals, and personal care products.
We previously reported the urinary concentrations of free plus conjugated species of several parabens in a convenience sample of 100 adults during 2003–2005 (Ye et al. 2006a). The frequencies of detection were comparable among the sample (MP, 99%; PP, 96%; EP, 58%; BP, 69%) and NHANES 2005–2006 (MP, 99.1%; PP, 92.7%; EP, 42%; BP, 47%). In addition, the median concentrations were practically the same for PP, slightly higher for MP, and lower for BP and EP in the NHANES 2005–2006 sample compared with the convenience population. Although exposure to these parabens (assessed by the urinary concentrations) may have changed, these two data sets are not directly comparable for establishing temporal exposure trends because of the small sample size and lack of national representativeness of the convenience sample. Nonetheless, both data sets confirm considerable human exposure to parabens and could potentially be used to derive internal dose exposure estimates. Of interest, the convenience biomonitoring samples have already been used to estimate internal dose of parabens (Cowan-Ellsberry and Robison 2009). Unfortunately, data are lacking regarding human metabolism of parabens, particularly of the fraction of the paraben excreted in the urine as the parent paraben (free or conjugated) versus the fraction excreted as p-hydroxybenzoic acid. This information, including a better understanding of the possible differences in metabolism by exposure route in humans, is needed to adequately link paraben urinary biomarker measurements to exposure and to internal dose.
We report here for the first time the concentrations of parabens among children and adolescents. Our data confirm that exposure occurs at these younger ages. Further, the apparent lower exposure among the younger segments of this NHANES 2005–2006 population (reflected in lower urinary concentrations than for adults) is likely associated with lifestyle. For example, in general, adults are more likely to use pharmaceuticals and personal care products than are children. We observed a similar age pattern for monoethyl phthalate (Silva et al. 2004), a metabolite of diethyl phthalate (DEP) for which a primary source of exposure may be the routine use of personal care products (Duty et al. 2005).
Although parabens are nonpersistent chemicals that are excreted from the body within hours after exposure (Janjua et al. 2008), examination session used as a surrogate for the time of urine collection was not a significant factor in explaining the variance of the urinary concentrations of the parabens. In contrast, we observed important differences in concentrations on the basis of demographic characteristics. Specifically, the LSGM MP and PP concentrations were significantly greater among people in the high household income category than among those in the medium- and low-income categories (Table 5), suggesting that the use of pharmaceuticals and personal care products according to socioeconomic status may affect paraben exposure.
Of interest, we observed that LSGM concentrations of MP and PP were highly dependent upon sex, age, and race/ethnicity (Table 5, Figures 1 and 2), as we have reported for other compounds (or their metabolites) found in personal care products, such as DEP (Silva et al. 2004) and benzophenone-3 (Calafat et al. 2008a). The higher concentrations of MP and PP found among women than among men were likely attributable to women’s increased use of personal care products, such as cosmetics and lotions. Non-Hispanic black children and adolescents had LSGM concentrations of MP and PP that were higher than or very similar to the concentrations in non-Hispanic black adults; non-Hispanic blacks had much higher MP and PP concentrations than did the other two race/ethnicity groups, particularly among children, adolescents, and adults 20–59 years old. These differences may result from increased, continuous, or prolonged use of beauty, hair, and/or skin care products specifically marketed to this population in whom the use often begins at a young age. The less dramatic differences by race/ethnicity among older adults may be explained by increased use of pharmaceuticals regardless of race/ethnicity that may compensate for differences in personal care products use. Because MP and PP are also used in food products, we cannot rule out that potential differences in diet, should they exist, may have also contributed to the differences in urinary concentrations of MP and PP among the various demographic groups examined.
Identifying populations in the highest exposure category (i.e., with concentrations above the 95th percentile) is an important consideration for public health. Our data suggest that females, non-Hispanic blacks, and, to a lesser extent, Mexican Americans have higher exposures to MP and PP than do other demographic segments of the general population. Specifically, females and non-Hispanic blacks were more likely to exhibit concentrations of MP and PP above the 95th percentile than were males, non-Hispanic whites, or Mexican Americans. In particular, females were 3.2 times more likely than males, and non-Hispanic blacks were about 5 times more likely than non-Hispanic whites and 2.5 times more likely than Mexican Americans, to have MP concentrations above the 95th percentile. Mexican Americans were about twice as likely as non-Hispanic whites to present MP concentrations above the 95th percentile. Similarly, for PP, females were 4.2 times more likely than males, and non-Hispanic whites were 3.6 times less likely than non-Hispanic blacks and 2.56 times less likely than Mexican Americans, to have MP concentrations above the 95th percentile. The likelihood of presenting PP concentrations above the 95th percentile did not differ (p = 0.13) between Mexican Americans and non-Hispanic blacks. Age was not significantly associated with having concentrations above the 95th percentile for either MP or PP.
In summary, we found significant differences in concentrations of parabens across demographic groups, particularly those associated with sex and race/ethnicity. These data can be used to establish a nationally representative baseline assessment of exposure—a baseline against which the concentrations of these parabens in future populations can be compared in order to identify exposure trends. These NHANES 2005–2006 data may also be useful in a risk assessment of parabens if warranted by toxicologic or epidemiologic studies.
- Andersen FA. 2008. Final amended report on the safety assessment of methylparaben, ethylparaben, propylparaben, isopropylparaben, butylparaben, isobutylparaben, and benzylparaben as used in cosmetic products. Int J Toxicol 27:1–82.
- Bando H, Mohri S, Yamashita F, Takakura Y, Hashida M. 1997. Effects of skin metabolism on percutaneous penetration of lipophilic drugs. J Pharm Sci 86:759–761.
- Bergfeld WE, Belsito DV, Marks JG, Andersen FA. 2005. Safety of ingredients used in cosmetics. J Am Acad Dermatol 52:125–132.
- Calafat AM, Wong L-Y, Ye X, Reidy JA, Needham LL. 2008. Concentrations of the sunscreen agent benzophenone-3 in residents of the United States: National Health and Nutrition Examination Survey 2003–2004. Environ Health Perspect 116:893–897.
- Calafat AM, Ye X, Wong LY, Reidy JA, Needham LL. 2008. Urinary concentrations of triclosan in the US population: 2003–2004. Environ Health Perspect 116:303–307.
- CDC (Centers for Disease Control and Prevention). 2009a. General Documentation on Laboratory Data. General Information about the NHANES 2005–2006 Laboratory Methodology and Public Data Files. Available: http://www.cdc.gov/nchs/nhanes/nhanes2005-2006/lab05_06.htm [accessed 2 September 2009].
- CDC (Centers for Disease Control and Prevention). 2009b. National Health and Nutrition Examination Survey. Available: http://www.cdc.gov/nchs/nhanes/about_nhanes.htm [accessed 2 September 2009].
- Cowan-Ellsberry CE, Robison SH. 2009. Refining aggregate exposure: example using parabens. Regul Toxicol Pharmacol 55:321–329.
- Darbre PD. 2006. Environmental oestrogens, cosmetics and breast cancer. Best Practice Res Clin Endocrinol Metabol 20:121–143.
- Darbre PD, Aljarrah A, Miller WR, Coldham NG, Sauer MJ, Pope GS. 2004. Concentrations of parabens in human breast tumours. J Appl Toxicol 24:5–13.
- Darbre PD, Harvey PW. 2008. Paraben esters: review of recent studies of endocrine toxicity, absorption, esterase and human exposure, and discussion of potential human health risks. J Appl Toxicol 28:561–578.
- Duty SM, Ackerman RM, Calafat AM, Hauser R. 2005. Personal care product use predicts urinary concentrations of some phthalate monoesters. Environ Health Perspect 113:1530–1535.
- El Hussein S, Muret P, Berard M, Makki S, Humbert P. 2007. Assessment of principal parabens used in cosmetics after their passage through human epidermis-dermis layers (ex-vivo study). Exp Dermatol 16:830–836.
- Elder RL. 1984. Final report on the safety assessment of methylparaben, ethylparaben, propylparaben and butylparaben. J Am Coll Toxicol 3:147–209.
- Golden R, Gandy J, Vollmer G. 2005. A review of the endocrine activity of parabens and implications for potential risks to human health. Crit Rev Toxicol 35:435–458.
- Harvey PW, Darbre P. 2004. Endocrine disrupters and human health: could oestrogenic chemicals in body care cosmetics adversely affect breast cancer incidence in women? A review of evidence and call for further research. J Appl Toxicol 24:167–176.
- Harville HM, Voorman R, Prusakiewicz JJ. 2007. Comparison of paraben stability in human and rat Skin. Drug Metab Lett 1:17–21.
- Janjua NR, Frederiksen H, Skakkebaek NE, Wulf HC, Andersson AM. 2008. Urinary excretion of phthalates and paraben after repeated whole-body topical application in humans. Int J Androl 31:118–129.
- Jewell C, Ackermann C, Payne NA, Fate G, Voorman R, Williams FM. 2007. Specificity of procaine and ester hydrolysis by human, minipig, and rat skin and liver. Drug Metab Dispos 35:2015–2022.
- Jewell C, Bennett P, Mutch E, Ackermann C, Williams FM. 2007. Inter-individual variability in esterases in human liver. Biochem Pharmacol 74:932–939.
- Kiwada H, Awazu S, Hanano M. 1979. Study on the biological fate of paraben at the dose of practical usage in rat. 1. Metabolism and excretion of ethyl para-hydroxybenzoate (ethyl paraben) and para-hydroxybenzoic acid. J Pharmacobio Dyn 2:356–364.
- Kiwada H, Awazu S, Hanano M. 1980. The study on the biological fate of paraben at the dose of practical usage in rat. 2. The pharmacokinetic study on the blood-concentration after the administration of ethyl paraben or para-hydroxybenzoic acid. J Pharmacobio Dyn 3:353–363.
- National Toxicology Program. 2004. Butylparaben [CAS No. 94-26-8]. Final Review of Toxicological Literature. Available: http://ntp.niehs.nih.gov/files/Butylparaben.pdf [accessed 15 December 2009].
- National Toxicology Program. 2006. NTP Concept Document. Butylparaben. Available: http://ntp.niehs.nih.gov/files/ButylparabenConcept.pdf [accessed 15 December 2009].
- Prusakiewicz JJ, Ackermann C, Voorman R. 2006. Comparison of skin esterase activities from different species. Pharm Res 23:1517–1524.
- Sandborgh-Englund G, Adolfsson-Erici M, Odham G, Ekstrand J. 2006. Pharmacokinetics of triclosan following oral ingestion in humans. J Toxicol Environ Health A 69:1861–1873.
- SCCP (Scientific Committee on Consumer Products). 2005. SCCP/0874/05—Extended Opinion on Parabens, Underarm Cosmetics and Breast Cancer, Adopted by the SCCP by Written Procedure on 28 January 2005. Brussels:European Commission.
- SCCP (Scientific Committee on Consumer Products). 2008. Scientific Committee on Consumer Products Opinion on: Parabens COLIPA no. P82 Adopted by the SCCP during the 16th Plenary Meeting on 24 June 2008.; Brussels:European Commission.
- Silva MJ, Barr DB, Reidy JA, Malek NA, Hodge CC, Caudill SP, et al. 2004. Urinary levels of seven phthalate metabolites in the US population from the National Health and Nutrition Examination Survey (NHANES) 1999–2000. Environ Health Perspect 112:331–338.
- Soni MG, Carabin IG, Burdock GA. 2005. Safety assessment of esters of p-hydroxybenzoic acid (parabens). Food Chem Toxicol 43:985–1015.
- Taylor JK. 1987. Quality Assurance of Chemical Measurements. Chelsea, MI:Lewis Publishers.
- Ye XY, Bishop AM, Reidy JA, Needham LL, Calafat AM. 2006. Parabens as urinary biomarkers of exposure in humans. Environ Health Perspect 114:1843–1846.
- Ye XY, Kuklenyik Z, Bishop AM, Needham LL, Calafat AM. 2006. Quantification of the urinary concentrations of parabens in humans by on-line solid phase extraction-high performance liquid chromatography-isotope dilution tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci 844:53–59.
2015 Impact Factor
EHP is pleased to announce its new impact factor of 8.44, up from 7.98 last year. We thank our authors, associate editors, reviewers, and readers for their contributions and support.
CEHN July 2016 Article of the Month
“Childhood Blood Lead Levels and Symptoms of Attention Deficit Hyperactivity Disorder (ADHD): A Cross-Sectional Study of Mexican Children” (doi:10.1289/ehp.1510067) has been selected by the Children’s Environmental Health Network (CEHN) as its July 2016 Article of the Month. These CEHN summaries discuss the potential policy implications of current children’s environmental health research.
Sign Up to Receive E-mail Alerts
Recent Advance Publications
Mortality due to Vegetation-Fire Originated PM2.5 Exposure in Europe – Assessment for the Years 2005 and 2008
Long-Term Exposure to Traffic-Related Air Pollution and Risk of Incident Atrial Fibrillation: A Cohort Study
IL-33 Drives Augmented Responses to Ozone in Obese Mice
Ambient Air Pollutant Exposures and Hospitalization for Kawasaki Disease in Taiwan: A Case-Crossover Study (2000-2010)
Relative Contributions of Agricultural Drift, Para-Occupational, and Residential Use Exposure Pathways to House Dust Pesticide Concentrations: Meta-Regression of Published Data