Children's Health Volume 120 | Issue 10 | October 2012
Rice Consumption and Urinary Arsenic Concentrations in U.S. Children
Matthew A. Davis,1 Todd A. Mackenzie,2 Kathryn L. Cottingham,3 Diane Gilbert-Diamond,2 Tracy Punshon,3 and Margaret R. Karagas2
Background: In adult populations, emerging evidence indicates that humans are exposed to arsenic by ingestion of contaminated foods such as rice, grains, and juice; yet little is known about arsenic exposure among children.
Objectives: Our goal was to determine whether rice consumption contributes to arsenic exposure in U.S. children.
Methods: We used data from the nationally representative National Health and Nutrition Examination Survey (NHANES) to examine the relationship between rice consumption (measured in 0.25 cups of cooked rice per day) over a 24-hr period and subsequent urinary arsenic concentration among the 2,323 children (6–17 years of age) who participated in NHANES from 2003 to 2008. We examined total urinary arsenic (excluding arsenobetaine and arsenocholine) and dimethylarsinic acid (DMA) concentrations overall and by age group: 6–11 years and 12–17 years.
Results: The median [interquartile range (IQR)] total urinary arsenic concentration among children who reported consuming rice was 8.9 μg/L (IQR: 5.3–15.6) compared with 5.5 μg/L (IQR: 3.1–8.4) among those who did not consume rice. After adjusting for potentially confounding factors, and restricting the study to participants who did not consume seafood in the preceding 24 hr, total urinary arsenic concentration increased 14.2% (95% confidence interval: 11.3, 17.1%) with each 0.25 cup increase in cooked rice consumption.
Conclusions: Our study suggests that rice consumption is a potential source of arsenic exposure in U.S. children.
Citation: Davis MA, Mackenzie TA, Cottingham KL, Gilbert-Diamond D, Punshon T, Karagas MR. 2012. Rice Consumption and Urinary Arsenic Concentrations in U.S. Children. Environ Health Perspect 120:1418–1424; http://dx.doi.org/10.1289/ehp.1205014
Address correspondence to M.R. Karagas, Section of Biostatistics and Epidemiology, Geisel School of Medicine at Dartmouth, One Medical Center Drive, 7927 Rubin Building, Lebanon, NH 03756 USA. Telephone: (603) 653-9010. Fax: (603) 653-9093. E-mail: firstname.lastname@example.org
We thank D. Miller and P. Villanueva at the U.S. Environmental Protection Agency (EPA) Office of Pesticide Programs for assistance with data from the Food Commodity Intake Database. We also thank K. Caldwell from the Centers for Disease Control and Prevention for her valuable suggestions.
This work was supported financially by grant P20 ES018175 from the National Institute of Environmental Health Sciences (NIEHS), National Institutes of Health (NIH); grant R25CA134286 from the National Cancer Institute (NCI), NIH; and RD-83459901 from the U.S. EPA. M.A.D. was supported by Award K01AT006162 from the NIH. The NIEHS, NIH, NCI, and U.S. EPA were not involved in the design and conduct of the study or collection, management, analysis, and interpretation of the data.
The contents of this manuscript are solely the responsibility of the authors and do not necessarily represent the official views of the NIEHS, NIH, NCI, or U.S. EPA. Further, the U.S. EPA does not endorse the purchase of any commercial products or services mentioned in the publication.
The authors declare they have no actual or potential conflicts of interest.
Received: 25 January 2012
Accepted: 31 July 2012
Advance Publication: 24 September 2012
Final Publication: 1 October 2012
- Supplemental Material (135 KB) PDF
Arsenic is a ubiquitous metalloid found as organic and inorganic forms in nature. Although the toxicity of inorganic arsenic is well established, arsenobetaine (from fish) is considered essentially nontoxic because it passes through the body unmetabolized (Cullen and Reimer 1989; Edmonds and Francesconi 1993; Ma and Le 1998; Navas-Acien et al. 2011); other organic forms such as dimethylarsenate, arsenolipids, and arsenosugars have uncertain toxicity. Emerging evidence indicates the potential for adverse health effects from inorganic arsenic exposure at the relatively low exposure levels common to populations worldwide, including an increased risk of cancer, cardiovascular and respiratory conditions, and diabetes mellitus (including gestational diabetes) (Amaral et al. 2011; Ettinger et al., 2009; European Food Safety Authority 2009; Karagas et al. 2001, 2004; Leonardi et al. 2012; Navas-Acien et al. 2011; Sohel et al. 2009). Additionally, studies of highly exposed populations have related childhood inorganic arsenic exposure to onset of cancers and lung disease later in life (Liaw et al. 2008; Smith et al. 2006; Yorifuji et al. 2011). The developing fetus and infants may be particularly susceptible to the adverse effects of inorganic arsenic (Hall et al. 2009; Vahter 2008). More specifically, fetal exposure to inorganic arsenic has been associated with low birth weight, increased risk of infection, and higher infant mortality (Rahman et al. 2009, 2010, 2011) in more highly exposed populations. In these populations, arsenic exposure during childhood has been associated with neurobehavioral effects in cross-sectional and prospective studies (Hamadani et al. 2011; Tsai et al. 2003; von Ehrenstein et al. 2007; Wasserman et al. 2007, 2011). Little is known about the possible long-term effects of persistent low-level arsenic exposure in children.
Contaminated drinking water is a well-recognized source of inorganic arsenic (Bhattacharya et al. 2002); however, diet is the primary exposure route for people with limited exposure via drinking water (European Food Safety Authority 2009). To date, dietary exposure to inorganic arsenic in children has generally been estimated from dietary patterns and measured arsenic concentrations in food (Bastias et al. 2010; European Food Safety Authority 2009; Martorell et al. 2011; Meacher et al. 2002b; Xue et al. 2010; Yost et al. 2004). In particular, it has been estimated that children < 3 years of age have the greatest exposures to inorganic arsenic primarily due to dietary sources such as rice consumption (European Food Safety Authority 2009; World Health Organization 2011).
Rice, grains, fruits, and juices are considered the primary food sources of arsenic exposure (Meacher et al. 2002a; Xue et al. 2010; Yost et al. 2004). Both inorganic and organic forms of arsenic accumulate in rice (Mitani et al. 2009) through the silicon transport system (Ma et al. 2008) because arsenous acid (the predominant form of arsenic in flooded rice paddies) is indistinguishable from silicic acid to the rice plant. Rice cultivars show wide variation in their ability to accumulate arsenic (3- to 37-fold) (Norton et al. 2012), and the proportion of inorganic arsenic in the grain also differs according to variety (Batista et al. 2011; Williams et al. 2005, 2007). Rice grown in the United States has been shown to contain higher amounts of total arsenic and a lower proportion of inorganic arsenic [and higher organic arsenic in the form of dimethylarsinic acid (DMA)] than rice from other countries (Meharg et al. 2009; Williams et al. 2005).
Although the United States population generally consumes less rice than those of other countries, consumption has increased (Batres-Marquez et al. 2009), and processed rice products such as flours and syrups are widely used. Among the roughly one-quarter of Americans who report rice consumption, the average amount of rice consumed is approximately 1 cup of cooked rice per day (Batres-Marquez et al. 2009). Daily rice consumption varies by level of education and race/ethnicity, with the greatest rice consumption among groups other than non-Hispanic whites, particularly those of Asian descent (Batres-Marquez et al. 2009). In addition, products such as rice cereal are often the first solid foods introduced at infancy (Jackson et al. 2012).
Ingested inorganic arsenic is excreted via the kidneys within a few days of ingestion as inorganic arsenic and methylated metabolites such as monomethylarsonic acid (MMA) and DMA. Considering that methylated species also can be present in food, excreted methylated forms could represent ingestion of these moieties as well as metabolism of ingested inorganic arsenic (Molin et al. 2011; European Food Safety Authority 2009). Urinary concentration of arsenic is regarded as a valid measure of recent exposure, especially compared with methods that rely on models of exposure from dietary information (Orloff et al. 2009). Rice consumption has been related to urinary arsenic concentrations in adults (Agusa et al. 2009; Cascio et al. 2011; Cleland et al. 2009; Gilbert-Diamond et al. 2011; He and Zheng 2010; Samal et al. 2011). However, to our knowledge, the relationship between rice consumption and urinary arsenic concentrations in children has not been evaluated directly. Therefore, we examined rice as a source of arsenic exposure in U.S. children by using data on rice consumption over the 24-hr period preceding measurement of urinary arsenic concentration in the National Health and Nutrition Examination Survey (NHANES).
We analyzed data from the NHANES survey years 2003–2008. The NHANES is a nationally representative multistage random survey of the noninstitutionalized U.S. population that is conducted by the U.S. National Center for Health Statistics. Information is gathered on health status and health behaviors through in-person interviews and detailed information is collected on diet. NHANES participants also undergo a clinical examination that includes laboratory measures such as blood and urine analyses. For this study, we used data from the NHANES demographics, in-person dietary questionnaire, physical examination, and laboratory and health questionnaire files. Because our study used publicly available and de-identified data, it was determined to be exempt from institutional board review by Dartmouth College’s Committee for the Protection of Human Subjects.
Study population. We analyzed data from all children (< 18 years of age) who participated in the NHANES survey from 2003 through 2008. During this period, 13,208 children participated in the NHANES, and the response rate for the entire survey was 76%. For each NHANES survey, samples from approximately one-third of the participants were randomly selected for urinary arsenic measurements. From 2003 through 2008, 2,477 children (6–17 years of age) had urinary arsenic concentrations measured in the NHANES. Of these, we excluded 154 children because of incomplete dietary information from the 24-hr recall; this yielded a final sample of 2,323 children for our study.
Urinary arsenic assessment. For NHANES, urine was collected from participants in arsenic-free containers and shipped on dry ice to the Environmental Health Sciences Laboratory at the National Center for Environmental Health (NCEH; Atlanta, GA) (Caldwell et al. 2009). At NCEH, urine samples were stored frozen (≤ –70°C) and analyzed within 3 weeks of collection following standardized protocols (Aposhian and Aposhian 2006; NCHS 2004). Total urinary arsenic concentrations were measured using inductively coupled plasma dynamic reaction cell–mass spectrometry on an ELAN DRC II ICPMS or Perkin-Elmer ELAN 6100 DRC plus (PerkinElmer SCIEX, Concord, ON, Canada); arsenic species and metabolites (arsenous acid, arsenic acid, MMA, DMA, arsenobetaine, and arsenocholine) were measured using high performance liquid chromatography (HPLC).
Method detection limits and interassay coefficients of variation (CV) varied among analytes and surveys. For total arsenic, the detection limit was 0.6 μg/L for the 2003–2004 survey and 0.7 μg/L for the 2005–2006 and 2007–2008 surveys. From 2003 to 2008, the detection limit was 1.7 μg/L for DMA, 0.9 μg/L for MMA, 1.2 µg/L for arsenous acid, 1.0 µg/L for arsenic acid, 0.6 µg/L for arsenocholine, and 0.4 μg/L for arsenobetaine. CV across NHANES lots varied from 3.0% to 6.1% for mean total arsenic concentrations, from 3.3% to 6.6% for DMA, and from 5.3% to 7.3% for arsenobetaine.
We focused our analyses on total urinary arsenic and urinary DMA concentrations becasue these were detected in most subjects. Urinary measurements of total arsenic and DMA, samples with levels below the detection limit (0.6% n = 13 and 1.0% n = 240, respectively) were assigned the value of the detection limit divided by the square root of 2 (Caldwell et al. 2009; Jones et al. 2011; Navas-Acien et al. 2008; Steinmaus et al. 2009). Due to uncertain or negligible health impacts of arsenobetaine and arsenocholine concentrations, we subtracted these components from the total urinary arsenic concentrations. For arsenobetaine, 48% of samples (n = 1,109) fell below the corresponding detection limit and were assigned the detection limit divided by the square root of 2. However, because only 23 participants (1.0%) had arsenocholine measures above the detection limit, we assigned a value of 0 to all measures that fell below the detection limit (Steinmaus et al. 2009). Thus, our definition of total arsenic included arsenous acid, arsenic acid, MMA, and DMA, consistent with previous studies (e.g., Gilbert-Diamond et al. 2011). Arsenous acid, arsenic acid, and MMA were not considered separately due to the low levels of detection (only 6.9%, 7.8%, and 40.9% of our study had values above the detection limit, respectively).
24-hr rice consumption. The in-person dietary questionnaire of NHANES collects detailed information on the study participant’s diet for the 24-hr period preceding the clinical and laboratory examinations (including urinary measurements) and for some measures (such as seafood consumption) up to a 30-day recall period. The NHANES 24-hr recall period is a validated assessment of dietary consumption (Moshfegh et al. 2008). At the examination, NHANES participants were asked to recall everything they ate and drank in the prior 24 hr, and NHANES staff coded these data and recorded information on the serving size. For children < 12 years of age, the dietary component was conducted with the assistance of a proxy (i.e., a parent or other caregiver), and for children 12–17 years of age the survey was administered without the assistance of a proxy.
We used U.S. Department of Agriculture (USDA) food codes to identify rice consumed during the in-person 24-hr recall period and to classify children as “rice eaters” versus “non-rice eaters.” As in previous studies, all food data from the 24-hr dietary recall period were matched to the Food Commodity Intake Database (FCID) (USDA 2010) to quantify exposure to rice (Batres-Marquez et al. 2009). The FCID provides conversion data to estimate the total content of food commodities such as rice, tomatoes, beans, and the like in each item with a USDA food code. We estimated the total amount of dry grams of rice consumed by each participant by multiplying the quantity of each food consumed during the 24-hr recall period by the FCID estimate of dry rice content (grams of rice per 100 g of food) for that specific food, and then summing across all foods consumed during the 24-hr recall period. To classify those children who consumed rice (rice eaters) versus those who did not (non-rice eaters), we operationally defined a rice eater as someone who consumed at least 0.25 cup of cooked rice (equivalent to 14.1 g white rice dry weight) in the 24-hr recall period (Batres-Marquez et al. 2009).
Other data. We also collected data on sociodemographics (age, sex, race/ethnicity, educational status, family income), body mass index (BMI; kilograms per meter squared), exposure to cigarette smoke, drinking-water source, and seafood consumption (obtained from both the 24-hr recall period and 30-day food recall questions). We estimated the percentages of the population that were normal weight, overweight, and obese by converting measured BMI to percentiles based on age and sex (< 85th percentile, normal; 85th to < 95th percentile, overweight; and ≥ 95th percentile, obese) (Centers for Disease Control and Prevention 2010). We anticipated that race/ethnicity would be related to rice consumption and therefore classified race/ethnicity as non-Hispanic white, non-Hispanic black, Mexican American, and other, multiple races. Due to the small number of individuals, the “other Hispanics” NHANES category was combined with the “other/multiple” race/ethnicity.
Because cigarette smoke is a potential source of arsenic exposure (Chen et al. 2004), we used serum cotinine to estimate passive or active exposure to cigarette smoke. The NHANES measures serum cotinine using an isotope-dilution HPLC/atmospheric pressure chemical ionization mass spectrometry method. For values below the detection limit of 0.015 ng/mL, a value of the detection limit divided by the square root of 2 was assigned (Jones et al. 2011; Navas-Acien et al. 2008).
We used urinary creatinine to account for urinary dilution (Barr et al. 2005). In the 2003–2004 and 2005–2006 NHANES surveys, urinary creatinine was measured on a Beckman Synchron CX3 (Beckman Coulter Inc., Brea, CA) using a Jaffe reaction, and in the 2007–2008 panel it was measured on a Roche/Hitachi Modular P (Roche Diagnostics Corp, Indianapolis, IN) using an enzymatic method. Therefore, we adjusted 2003–2004 and 2005–2006 urinary creatinine measurements to 2007–2008 equivalents (Gebel 2002; NCHS 2009).
In the United States, arsenic exposure through drinking water is found primarily in private unregulated water systems (Karagas et al. 2000; Nuckols et al. 2011). Although we were unable to obtain measurements of arsenic in drinking water for NHANES participants, we used their self-reported drinking-water source to estimate potential exposure as either public (using a community water source) or private (defined as either a well, spring, or cistern water source) water.
To exclude the possibility that seafood contributed to forms of arsenic exposure other than arsenobetaine or arsenocholine, such as DMA, we used the USDA food codes that correspond with fish, shellfish, mollusks, and/or crustaceans to identify children who consumed any seafood during the 24-hr recall period in our primary analyses [see Supplemental Material, Table S1 (http://dx.doi.org/10.1289/ehp.1205014)] (Navas-Acien et al. 2011). Furthermore, because seafood consumption may affect urinary arsenic concentration for up to 3 days, we also performed a secondary analysis in which we restricted our sample to children who reported no seafood consumption in the 30 days before urinary arsenic measurement (Molin et al. 2011) (see Supplemental Material, Table S2).
Statistical analyses. The NHANES uses a stratified sampling methodology that makes it possible to derive national estimates from survey participants’ data. To account for this sampling, we used complex survey design methods in Stata version 12.0 (StataCorp., College Station, TX) for all analyses. These methods account for a respondent’s probability of selection and for the NHANES sampling methodology by calculating weighting factors for each respondent that account for sampling strata, primary sampling units, and person weight variables (NCHS 2005). For all analyses we set the p-value for statistical significance to 0.05 (2-sided).
Because metabolic processes may vary according to a child’s age (Hall et al. 2009) and NHANES dietary data were collected differently according to age (i.e., with and without a parent or caregiver), we stratified our sample into age groups of 6–11 years and 12–17 years. Analyses were performed on all ages as well as according to these two age categories.
We log10-transformed total urinary arsenic (the original NHANES total arsenic measure minus arsenobetaine and arsenocholine) and urinary DMA concentrations. This transformation produced a linear association with rice consumption for total urinary arsenic (lack-of-fit p-value = 0.45; Draper and Smith 1981), and improved the homoskedasticity and normality of model residuals, for both total urinary arsenic and urinary DMA. For this lack-of-fit test, the null hypothesis is that there is no bias—that bias error and pure error are approximately the same; the null hypothesis thus is rejected when an F-statistic comparing bias error to pure error exceeds a critical value. The exponentiated model coefficients represent the relative (percent) change in the dependent variable from its mean value at the reference level of exposure (Vittinghoff et al. 2005).
Urinary creatinine can be a strong predictor of arsenic methylation efficiency; thus, we included it as an independent variable in our multiple regression models (Barr et al. 2005). However, analyses with and without creatinine yielded similar results. We adjusted for potential confounding using three different models. Our baseline adjustment model (model 1) included age (continuous), sex (boy/girl), race/ethnicity (non-Hispanic white/non-Hispanic black/Mexican American/other, multiple races), and urinary creatinine concentration (continuous). Additionally, we fit a model that further adjusted for BMI (as a continuous variable) and serum cotinine concentration (continuous) (model 2). The final model additionally adjusted for water source (public/private) and was restricted to only those children who reported no seafood consumption during the 24-hr recall period (model 3). As a secondary analysis, we repeated model 3 restricting the model to children who reported no seafood consumption in the 30-day food recall questions [see Supplemental Material, Table S2 (http://dx.doi.org/10.1289/ehp.1205014].
We used rice consumption as a predictor variable in two ways. First, we treated rice consumption as a dichotomous variable, evaluating selected population characteristics and urinary arsenic variables (total arsenic and DMA urinary concentrations) according to whether the study participant consumed ≥ 0.25 cup of cooked rice during the 24-hr recall period. To compare the characteristics of study participants we used a chi-square test for categorical variables. We then explored the potential dose–response relationship between 0.25 cup cooked rice consumed during the 24-hr recall period and log10-transformed total arsenic and DMA using multiple linear regression as described.
Study participants’ characteristics according to rice eater status. Approximately 20% (471 of 2,323 study participants) of children in our sample reported consuming at least 0.25 cup of cooked rice in the 24 hr before urinary arsenic measurement. Among children who consumed rice, total cups of cooked rice consumed per day varied from 0.25 to 3.9 cups, with a mean of 0.8 cups. Characteristics that differed according to rice consumption status included race/ethnicity and seafood consumption status (Table 1). Children who consumed rice were less likely to be non-Hispanic white (44.0% vs. 63.7%) and more likely to be classified as “other, multiple races” (23.5% vs. 9.8%) (overall p-value < 0.01). Rice eaters were nearly twice as likely as non-rice eaters to report having consumed at least one form of seafood during the 24-hr recall period (14.0% vs. 7.1%, p-value < 0.01). Overall, 66.5% of rice eaters reported having consumed seafood in the 30-day food recall questions vs. 57.6% among non-rice eaters (p-value = 0.03) [see Supplemental Material, Table S3 (http://dx.doi.org/10.1289/ehp.1205014]. Cotinine level also differed according to rice eater status. Among non-rice eaters 44.8% of children had serum cotinine levels ≥ 10 ng/mL vs. 36.7% among rice eaters (overall p-value = 0.07).
Urinary arsenic concentration according to rice eater status. Total urinary arsenic (excluding arsenobetaine and arsenocholine) and urinary DMA concentrations were higher among children who reported consuming ≥ 0.25 cup rice during the 24 hr preceding urinary arsenic measurement compared with those who did not, overall, and stratified by age and by those reporting seafood consumption (Table 2). The median (interquartile range) of total urinary arsenic concentration among children who reported consuming rice was 8.9 μg/L [interquartile range (IQR): 5.3–15.6] compared with 5.5 μg/L (IQR: 3.1–8.4) among those who did not consume rice. Urinary DMA among rice eaters was nearly twice that of non-rice eaters [median, 6.0 μg/L (IQR: 3.7–10.0) compared with 3.6 μg/L (IQR: 2.1–5.1), respectively].
Urinary arsenic concentration and amount of estimated rice consumption. Log10-transformed urinary total arsenic and DMA increased with rice consumption (Table 3). In the models adjusted for participant characteristics, serum cotinine, and urinary creatinine concentration (model 1), each 0.25 cup of rice consumption was associated with a 14.3% [95% confidence interval (CI): 10.2, 18.5%] and 13.5% (95% CI: 10.3, 16.9%) increase in urinary total arsenic and DMA concentration, respectively. Estimates from models further adjusted for BMI, cotinine, and water source and restricted to children who did not report seafood consumption were similar (Table 3).
Estimates for the effects of rice consumption on total urinary arsenic concentration differed by age category (ages 6–11 years vs. 12–17 years) (Table 3). Each 0.25 cup of rice consumption was associated with a 22.0% (95% CI: 15.7, 28.7%) increase in total urinary arsenic among children in the younger age category compared with a 10.7% (95% CI: 6.6, 14.9%) increase among those in the older age category in model 1. Differences in the estimated effect of rice consumption between the age categories persisted in our other models, albeit attenuated in model 3. In our secondary analyses, restricted to only children who reported no seafood consumption in the preceding 30 days, we obtained similar results [see Supplemental Material, Table S2 (http://dx.doi.org/10.1289/ehp.1205014). Estimates for urinary DMA in relation to rice consumption also differed by age category (Table 3). Rice consumption was associated with a 19.9% (95% CI: 14.7, 25.3%) increase in urinary DMA among children in the younger age category compared with a 10.7% (95% CI: 7.5, 14.0%) increase among those in the older age category. However, this age group difference was less apparent in model 3 (14.7%; 95% CI: 10.5, 19.0% vs. 12.5%; 95% CI: 8.7, 16.4% respectively).
Urinary arsenic concentration and other factors. In the multiple regression models excluding those who reported seafood in the prior 24 hr, rice consumption was the strongest independent predictor of total urinary arsenic concentration (Table 4). Both age and urinary creatinine also were statistically significant predictors of total urinary arsenic concentration. Each 1-year increase in age was associated with a 5.2% decrease (95% CI: –6.9, –3.6%) in total urinary arsenic concentration. Total urinary arsenic concentration varied by race/ethnicity, but the association was statistically significant only for Mexican Americans (13.0% higher than non-Hispanic whites; 95% CI: 1.0, 26.5%). Each increase in nanograms per milliliter of serum cotinine level was associated with a 0.7% increase (95% CI: 0.6, 0.8%) in total urinary arsenic concentration.
In this nationally representative study of U.S. children, we found that urinary arsenic concentrations—a biomarker of recent arsenic exposure—were associated with reported rice consumption in the 24 hr before urine collection. These findings are consistent with other recent studies that have examined rice as a source of dietary exposure to arsenic in adult populations (Agusa et al. 2009; Cascio et al. 2011; Cleland et al. 2009; He and Zheng 2010; Samal et al. 2011). In the United States, studies of specific populations have reported more than three times the national average of 6.0 μg/L in high-rice-consuming individuals (i.e., Korean Americans) (Cleland et al. 2009). Also, findings from this study of children corroborate those of our previous study of rice consumption and urinary arsenic concentrations among pregnant women in the United States (Gilbert-Diamond et al. 2011).
At present, the health effects of low-level arsenic exposure are uncertain, especially in children, and no studies have specifically evaluated the potential health effects of arsenic in rice to our knowledge. Previous studies have associated childhood exposure to high levels of inorganic arsenic, primarily from drinking water, with numerous adverse health effects, including neurobehavioral effects such as reduced vocabulary and object assembly skills (von Ehrenstein et al. 2007), attention and memory (Tsai et al. 2003), and intelligence (Wasserman et al. 2004). However, it is currently unknown whether low levels of arsenic exposure, or exposure from arsenic intake via rice specifically, have similar effects.
Rice consumption varies among individuals and among subgroups of the population. Higher rice consumption among racial/ethnic minorities such as those of Asian descent and populations with lower income and less education has been reported previously (Batres-Marquez et al. 2009), consistent with patterns observed in our study of children who participated in NHANES. Rice and rice products may constitute an appreciable portion of the diet in young children (World Health Organization 2011) and among people on wheat-free diets [e.g., celiac disease patients (Ludvigsson and Green 2011)]. As we reported recently, certain toddler formulas containing brown rice syrup had relatively high concentrations of arsenic (Jackson et al. 2012). Thus, measurement of biomarkers in children may help us determine common sources of arsenic exposure, such as via rice and rice products, and whether these sources pose a health risk.
A number of limitations of our study must be acknowledged. First, seafood (including fish, shellfish, mollusks, and/or crustaceans) is a well-recognized source of arsenic, particularly the organic forms such as arsenobetaine, arsenosugars, arsenolipids, and DMA (Navas-Acien et al. 2011). To minimize the effect of arsenic ingestion from seafood, we restricted a subset of our analyses (model 3) to children who reported no seafood consumption of any kind during the 24-hr recall period, and performed secondary analyses restricted to children who did not consume seafood in the previous 30 days [see Supplemental Material, Table S2 (http://dx.doi.org/10.1289/ehp.1205014]. However, our results were robust to this more conservative exclusion of seafood eaters for up to 30 days before urinary measurement.
Second, as mentioned, our estimates of rice consumption do not directly translate into estimates of arsenic consumed because of the large variation in arsenic concentrations in rice (Batista et al. 2011; Williams et al. 2005, 2007). Collecting information on the type of rice consumed (e.g., brown, white, or other varieties) would allow for better estimation of the association between rice intake and arsenic exposure in the future. Such information further could improve our understanding of the extent to which the DMA in urine results from the metabolism of inorganic arsenic versus the excretion of DMA from rice itself; these two pathways are indistinguishable in our study.
Third, we likely underestimated rice consumption. The addition of a diverse range of rice products to processed foods makes it difficult to accurately assess total rice consumption in the United States. Rice bran, rice flour, rice starch, and rice syrup are often added to products, including breakfast cereals aimed at children, cereal bars, and gluten-free products, and these products may contain arsenic (Jackson et al. 2012). Therefore, our estimates of the relationship between urinary arsenic concentrations and rice consumption are likely to be conservative because of errors in our estimate of rice consumption itself, which could have biased our parameter estimates towards the null.
Despite these limitations, our findings suggest that rice is a potential source of arsenic exposure in U.S. children and highlight the need to better understand the health consequences of common levels of arsenic exposure early in life.
Agusa T, Kunito T, Minh TB, Kim Trang PT, Iwata H, Viet PH, et al. 2009. Relationship of urinary arsenic metabolites to intake estimates in residents of the Red River Delta, Vietnam. Environ Pollut 157(2):396–403.
Amaral AF, Porta M, Silverman DT, Milne RL, Kogevinas M, Rothman N, et al. 2011. Pancreatic cancer risk and levels of trace elements. Gut; doi: 10.1136/gutjnl-2011-301086
Barr DB, Wilder LC, Caudill SP, Gonzalez AJ, Needham LL, Pirkle JL. 2005. Urinary creatinine concentrations in the U.S. population: implications for urinary biologic monitoring measurements. Environ Health Perspect 113:192–200.
Bastias JM, Bermudez M, Carrasco J, Espinoza O, Munoz M, Galotto MJ, et al. 2010. Determination of dietary intake of total arsenic, inorganic arsenic and total mercury in the Chilean school meal program. Food Sci Technol Int 16(5):443–450.
Batista BL, Souza JM, De Souza SS, Barbosa F Jr. 2011. Speciation of arsenic in rice and estimation of daily intake of different arsenic species by Brazilians through rice consumption. J Hazard Mater 191(1–3):342–348.
Caldwell KL, Jones RL, Verdon CP, Jarrett JM, Caudill SP, Osterloh JD. 2009. Levels of urinary total and speciated arsenic in the US population: National Health and Nutrition Examination Survey 2003–2004. J Expo Sci Environ Epidemiol 19(1):59–68.
Centers for Disease Control and Prevention. 2010. About BMI for Children and Teens. Available: http://www.cdc.gov/healthyweight/assessing/bmi/childrens_bmi/about_childrens_bmi.html [accessed 10 December 2011].
Chen CL, Hsu LI, Chiou HY, Hsueh YM, Chen SY, Wu MM, et al. 2004. Ingested arsenic, cigarette smoking, and lung cancer risk: a follow-up study in arseniasis-endemic areas in Taiwan. JAMA 292(24):2984–2990.
Cleland B, Tsuchiya A, Kalman DA, Dills R, Burbacher TM, White JW, et al. 2009. Arsenic exposure within the Korean community (United States) based on dietary behavior and arsenic levels in hair, urine, air, and water. Environ Health Perspect 117:632–638.
Ettinger AS, Zota AR, Amarasiriwardena CJ, Hopkins MR, Schwartz J, Hu H, Wright RO. 2009. Maternal arsenic exposure and impaired glucose tolerance during pregnancy. Environ Health Perspect 117:1059–1064.
European Food Safety Authority. 2009. EFSA Panel on Contaminants in the Food Chain (CONTAM): Scientific Opinion on Arsenic in Food. EFSA J 7(10):60–71. Available: http://www.efsa.europa.eu/en/efsajournal/pub/1351.htm [accessed 24 August 2012].
Hall MN, Liu X, Slavkovich V, Ilievski V, Pilsner JR, Alam S, et al. 2009. Folate, cobalamin, dysteine, homocysteine, and arsenic metabolism among children in Bangladesh. Environ Health Perspect 117:825–831.
Hamadani JD, Tofail F, Nermell B, Gardner R, Shiraji S, Bottai M, et al. 2011. Critical windows of exposure for arsenic-associated impairment of cognitive function in pre-school girls and boys: a population-based cohort study. Int J Epidemiol 40(6):1593–1604.
Jones MR, Tellez-Plaza M, Sharrett AR, Guallar E, Navas-Acien A. 2011. Urine arsenic and hypertension in US adults: the 2003–2008 National Health and Nutrition Examination Survey. Epidemiology 22(2):153–161.
Karagas MR, Stukel TA, Morris JS, Tosteson TD, Weiss JE, Spencer SK, et al. 2001. Skin cancer risk in relation to toenail arsenic concentrations in a US population-based case-control study. Am J Epidemiol 153(6):559–565.
Karagas MR, Tosteson TD, Blum J, Klaue B, Weiss JE, Stannard V, et al. 2000. Measurement of low levels of arsenic exposure: a comparison of water and toenail concentrations. Am J Epidemiol 152(1):84–90.
Karagas MR, Tosteson TD, Morris JS, Demidenko E, Mott LA, Heaney J, et al. 2004. Incidence of transitional cell carcinoma of the bladder and arsenic exposure in New Hampshire. Cancer Causes Control 15(5):465–472.
Leonardi G, Vahter M, Clemens F, Goessler W, Gurzau E, Hemminki K, et al. 2012. Arsenic and basal cell carcinoma in areas of Hungary, Romania, and Slovakia: a case–control study. Environ Health Perspect 120:721–726.
Liaw J, Marshall G, Yuan Y, Ferreccio C, Steinmaus C, Smith AH. 2008. Increased childhood liver cancer mortality and arsenic in drinking water in northern Chile. Cancer Epidemiol Biomarkers Prev 17(8):1982–1987.
Martorell I, Perello G, Marti-Cid R, Llobet JM, Castell V, Domingo JL. 2011. Human exposure to arsenic, cadmium, mercury, and lead from foods in Catalonia, Spain: temporal trend. Biol Trace Elem Res 142(3):309–322.
Meharg AA, Williams PN, Adomako E, Lawgali YY, Deacon C, Villada A, et al. 2009. Geographical variation in total and inorganic arsenic content of polished (white) rice. Environ Sci Technol 43(5):1612–1617.
Mitani N, Chiba Y, Yamaji N, Ma JF. 2009. Identification and characterization of maize and barley Lsi2-like silicon efflux transporters reveals a distinct silicon uptake system from that in rice. Plant Cell 21(7):2133–2142.
Moshfegh AJ, Rhodes DG, Baer DJ, Murayi T, Clemens JC, Rumpler WV, et al. 2008. The US Department of Agriculture Automated Multiple-Pass Method reduces bias in the collection of energy intakes. Am J Clin Nutr 88(2):324–332.
Navas-Acien A, Francesconi KA, Silbergeld EK, Guallar E. 2011. Seafood intake and urine concentrations of total arsenic, dimethylarsinate and arsenobetaine in the US population. Environ Res 111(1):110–118.
NCHS (National Center for Health Statistics). 2004. Laboratory Procedure Manual: Arsenobetaine, Arsenocholine, Trimethylarsine Oxide, Monomethylarsonic Acid, Dimethylarsinic Acid, Arsenous (III) Acid, Arsenic (V) Acid, Total Arsenic. Available: http://www.cdc.gov/nchs/data/nhanes/nhanes_07_08/uas_e_met_arsenic.pdf [accessed 7 December 2011].
NCHS (National Center for Health Statistics). 2005. Analytic and Reporting Guidelines. The National Health and Nutritional Examination Survey (NHANES). Hyattsville, MD:National Center for Health Statistics. Available: http://www.cdc.gov/nchs/data/nhanes/nhanes_03_04/nhanes_analytic_guidelines_dec_2005.pdf [accessed 10 December 2011].
NCHS (National Center for Health Statistics). 2009. 2007–2008 Data Documentation, Codebook, and Frequencies. Urinary Albumin and Urinary Creatinine (ALB_CR_E). Available: http://www.cdc.gov/nchs/nhanes/nhanes2007-2008/ALB_CR_E.htm [accessed 20 April 2012].
Norton GJ, Pinson SR, Alexander J, McKay S, Hansen H, Duan GL, et al. 2012. Variation in grain arsenic assessed in a diverse panel of rice (Oryza sativa) grown in multiple sites. New Phytol 193(3):650–664.
Rahman A, Vahter M, Ekstrom EC, Persson LA. 2011. Arsenic exposure in pregnancy increases the risk of lower respiratory tract infection and diarrhea during infancy in Bangladesh. Environ Health Perspect 119:719–724.
Rahman A, Vahter M, Smith AH, Nermell B, Yunus M, El Arifeen S, et al. 2009. Arsenic exposure during pregnancy and size at birth: a prospective cohort study in Bangladesh. Am J Epidemiol 169(3):304–312.
Samal AC, Kar S, Bhattacharya P, Santra SC. 2011. Human exposure to arsenic through foodstuffs cultivated using arsenic contaminated groundwater in areas of West Bengal, India. J Environ Sci Health A Tox Hazard Subst Environ Eng 46(11):1259–1265.
Smith AH, Marshall G, Yuan Y, Ferreccio C, Liaw J, von Ehrenstein O, et al. 2006. Increased mortality from lung cancer and bronchiectasis in young adults after exposure to arsenic in utero and in early childhood. Environ Health Perspect 114:1293–1296.
Sohel N, Persson LA, Rahman M, Streatfield PK, Yunus M, Ekstrom EC, et al. 2009. Arsenic in drinking water and adult mortality: a population-based cohort study in rural Bangladesh. Epidemiology 20(6):824–830.
USDA (U.S. Department of Agriculture). 2010. Food Commodity Intake Database. Available: http://www.ars.usda.gov/Services/docs.htm?docid=14514 [accessed 10 December 2011].
Wasserman GA, Liu X, Parvez F, Ahsan H, Factor-Litvak P, Kline J, et al. 2007. Water arsenic exposure and intellectual function in 6-year-old children in Araihazar, Bangladesh. Environ Health Perspect 115:285–289.
Wasserman GA, Liu X, Parvez F, Ahsan H, Factor-Litvak P, van Geen A, et al. 2004. Water arsenic exposure and children’s intellectual function in Araihazar, Bangladesh. Environ Health Perspect 112:1329–1333.
Williams PN, Price AH, Raab A, Hossain SA, Feldmann J, Meharg AA. 2005. Variation in arsenic speciation and concentration in paddy rice related to dietary exposure. Environ Sci Technol 39(15):5531–5540.
Williams PN, Raab A, Feldmann J, Meharg AA. 2007. Market basket survey shows elevated levels of As in south central U.S. processed rice compared to California: consequences for human dietary exposure. Environ Sci Technol 41(7):2178–2183.
World Health Organization. 2011. Safety Evaluation of Certain Contaminants in Food. Available: http://whqlibdoc.who.int/publications/2011/9789241660631_eng.pdf [accessed 28 April 2012].
For a number of years, EHP has posted accepted manuscripts online as what we termed Advance Publications. The concept here was simple—to get the content online as quickly as possible in an effort both to get authors the attention they deserve and to get readers the information they need. This product has served our community well, but it is not without its flaws. … MORE
EHP has launched an updated homepage! This bold, new look will give readers new ways to find our content, and will allow our Editors to highlight important articles that our audience needs to be aware of. … MORE
Featured Children’s Health
Birgit Claus Henn, Adrienne S. Ettinger, Marianne R. Hopkins, Rebecca Jim, Chitra Amarasiriwardena, David C. Christiani, Brent A. Coull, David C. Bellinger, and Robert O. Wright
Diane Gilbert-Diamond, Jennifer A. Emond, Emily R. Baker, Susan A. Korrick, and Margaret R. Karagas
Benjamin B. Green, Margaret R. Karagas, Tracy Punshon, Brian P. Jackson, David J. Robbins, E. Andres Houseman, and Carmen J. Marsit