Research Article Volume 121 | Issue 4 | April 2013
Environ Health Perspect; DOI:10.1289/ehp.1206367
Phthalate Concentrations and Dietary Exposure from Food Purchased in New York State
Arnold Schecter,1 Matthew Lorber,2 Ying Guo,3 Qian Wu,3,4 Se Hun Yun,3,4 Kurunthachalam Kannan,3,4 Madeline Hommel,1 Nadia Imran,1 Linda S. Hynan,5 Dunlei Cheng,1 Justin A. Colacino,6 and Linda S. Birnbaum7,8
Background: Phthalates have been found in many personal care and industrial products, but have not previously been reported in food purchased in the United States. Phthalates are ubiquitous synthetic compounds and therefore difficult to measure in foods containing trace levels. Phthalates have been associated with endocrine disruption and developmental alteration.
Objectives: Our goals were to report concentrations of phthalates in U.S. food for the first time, specifically, nine phthalates in 72 individual food samples purchased in Albany, New York, and to compare these findings with other countries and estimate dietary phthalate intake.
Methods: A convenience sample of commonly consumed foods was purchased from New York supermarkets. Methods were developed to analyze these foods using gas chromatography–mass spectroscopy. Dietary intakes of phthalates were estimated as the product of the food consumption rate and concentration of phthalates in that food.
Results: The range of detection frequency of individual phthalates varied from 6% for dicyclohexyl phthalate (DCHP) to 74% for di-2-ethylhexyl phthalate (DEHP). DEHP concentrations were the highest of the phthalates measured in all foods except beef [where di-n-octyl phthalate (DnOP) was the highest phthalate found], with pork having the highest estimated mean concentration of any food group (mean 300 ng/g; maximum, 1,158 ng/g). Estimated mean adult intakes ranged from 0.004 μg/kg/day for dimethyl phthalate (DMP) to 0.673 μg/kg/day for DEHP.
Conclusions: Phthalates are widely present in U.S. foods. While estimated intakes for individual phthalates in this study were more than an order of magnitude lower than U.S. Environmental Protection Agency reference doses, cumulative exposure to phthalates is of concern and a more representative survey of U.S. foods is indicated.
Citation: Schecter A, Lorber M, Guo Y, Wu Q, Yun SH, Kannan K, Hommel M, Imran N, Hynan LS, Cheng D, Colacino JA, Birnbaum LS. 2013. Phthalate Concentrations and Dietary Exposure from Food Purchased in New York State. Environ Health Perspect 121:473–479; http://dx.doi.org/10.1289/ehp.1206367
Address correspondence to A. Schecter, University of Texas School of Public Health, 6011 Harry Hines Blvd., V8.122E, Dallas, TX 75390-9128 USA. Phone: (214) 336-8519. E-mail: email@example.com
This study was funded by the Gustavus and Louise Pfeiffer Research Foundation and and funded in part by the intramural research program of the National Institutes of Health (NIH), National Cancer Institute (NCI). Support for J.A.C. was provided by training grants from the National Institute of Environmental Health Sciences (NIEHS), NIH (T32 ES007062), and the National Human Genome Research Institute (T32 HG00040).
The findings and conclusions in this report are those of the authors. This manuscript does not represent the views of the U.S. Environmental Protection Agency, the New York State Department of Health, the NCI, or the NIEHS, NIH.
The authors declare they have no actual or potential competing financial interests.
Received: 5 December 2012
Accepted: 15 February 2013
Advance Publication: 6 March 2013
Final Publication: 1 April 2013
- Supplemental Material (684 KB) PDF
Phthalates are diesters of phthalic acids, a class of industrial chemicals extensively used since the early 20th century as softeners of plastics, solvents in perfumes, and additives to hairsprays and lubricants and as insect repellents. Di-2-ethylhexyl phthalate (DEHP) is used primarily as a plasticizer for polyvinyl chloride (PVC) and can therefore be found in a variety of products such as floor and wall coverings, vinyl gloves, toys, child care articles, food packaging materials, and medical devices (Green et al. 2005). After absorption, the parent diester phthalates are rapidly hydrolyzed to the corresponding monoesters, some of which are then further metabolized, with the metabolites excreted in urine and feces. In humans, phthalates are eliminated mostly within hours, with excretion complete by a day or two; half-lives in the body are in hours (Koch and Calafat 2009). For phthalates with short alkyl chains, monoesters represent the major human metabolite, but in the case of phthalates with long alkyl chains, including DEHP, diisononyl phthalate (DINP) and diisodecyl phthalate (DIDP), the monoesters are further metabolized via ω- and ω-1-oxidation of the aliphatic side chain (Agency for Toxic Substances and Disease Registry 2002).
In animal studies, phthalates have been associated with a variety of deleterious health effects. Certain phthalates are reproductive toxicants, especially affecting the male reproductive system (Foster et al. 2000). In rats, phthalate exposure resulted in decreased testicular weight and seminiferous tubular atrophy (Kamrin 2009). Among adult men, urinary phthalate metabolite concentration has been associated with increased DNA damage in sperm (Duty et al. 2003). A possible association between premature breast development and early phthalate exposure in girls has been reported, based on a study comparing serum phthalate concentrations from 41 cases with 35 control samples (Colón et al. 2000). In women, shortened pregnancy has been associated with phthalate exposure; average gestational age at birth was significantly shorter in 65 newborns with detectable mono-2-ethylhexyl phthalate (MEHP) in cord serum compared with 19 newborns with MEHP-negative cord serum (Latini et al. 2003). Prenatal phthalate exposure is associated with a decrease in anogenital distance among male infants (Swan et al. 2005).
Existing exposure pathway assessments for phthalates have included consideration of food and water ingestion, soil and dust ingestion, dermal contact (personal care products, toys, textiles, gloves, paints/adhesives, and dust particles), and inhalation (indoor/outdoor air, hair/paint sprays). Phthalates can migrate into food through the production, packaging, and preparation of food. In a review of the literature, Cao and Xu-Liang (2010) identified sources of phthalates in food including PVC tubing used in food production, food-packaging films (also known as cling films), PVC gaskets in jars, printer inks on labels, and other sources. A recent and comprehensive exposure pathway assessment was conducted by Clark et al. (2011) using data from studies conducted in several countries. Food exposures were estimated primarily based on duplicate diet composite studies in which phthalates were measured in samples of all food and drink consumed by participants. For example, Fromme et al. (2007) collected duplicate diet samples for 50 individual participants 14–60 years of age over 7 days and measured them for several phthalates. Other duplicate diet studies include an analysis of 63 1-week hospital diet samples by Tsumura et al. (2001) and a study of 29 total diet samples and 11 baby food or infant formula samples by Petersen and Breindahl (2000). Duplicate diet studies provide the best data for overall dietary exposure assessment because they capture exposures from food as prepared and eaten. However, these studies are costly and usually test only a small number of individuals and thus may not be representative of the larger population in that country. A weakness of total diet studies is that they do not indicate the type of food products that are major contributors to contaminant exposures. On the other hand, analysis of individual food items for phthalates can provide such information. Total diet studies and also market basket surveys of phthalates in food, such as the one reported here, are rare. Some surveys have been conducted in Europe, Canada, and China [e.g., see Fierens et al. 2012; Food Standards Agency (FSA) 2012; Guo et al. 2012; Page and Lacroix 1995; Wormuth et al. 2006]; results of these studies are compared with the results of the present analysis.
Phthalate measurement in food is difficult because of the ubiquitous occurrence of phthalates in many products, including analytical solvents as well as laboratory air and equipment. Although some phthalate food concentration studies appear in the scientific literature, the results may suffer from the issue of ubiquitous contamination. To our knowledge, phthalate concentrations in foods from the United States have not previously been reported. The purpose of this study was to describe for the first time the presence and concentrations of phthalates in foods as purchased at U.S. supermarkets. In this study, we collected a convenience sample of 72 commonly consumed foods from supermarkets in Albany, New York, for the analysis of nine phthalate esters. Additional care was taken to insure that potential laboratory contamination would not influence the results, including the subtraction of blank concentrations from sample measurements [see Guo and Kannan (2012) for a review of contamination issues and how we resolved those concerns]. These issues are further addressed later in this report.
Sample collection. A convenience sample of 72 commonly consumed food samples [see Supplemental Material, Table S1 (http://dx.doi.org/10.1289/ehp.1206367)] was purchased from supermarkets in Albany, New York, in April 2011. Samples were frozen at –20°C after purchase. Among the 72 foods that were measured, 65 were grouped into ≥ 1 food categories (Table 1) and 7 were not included in any of the 13 food categories under consideration because they were mixtures and difficult to place in food categories (e.g., chicken pot pie, pizza, onion soup mix).
Analytical methods. Each sample was individually analyzed for nine phthalates: dimethyl phthalate (DMP), diethyl phthalate (DEP), butyl benzyl phthalate (BBzP), di-n-butyl phthalate (DBP), diisobutyl phthalate (DiBP), DEHP, di-n-hexyl phthalate (DnHP), dicyclohexyl phthalate (DCHP), and di-n-octyl phthalate (DnOP). All glassware was baked at 450°C overnight. A detailed report on the analysis and quality assurance and quality control methods is given elsewhere (Guo and Kannan 2012; Guo et al. 2012). All food samples (1.5–20 g), except soft drinks, were freeze-dried using a freeze drier (Labconco, Kansas City, MO), ground to a fine powder using a precleaned mortar and pestle, and spiked with 50 ng deuterated internal standards [D4-DMP, D4-DEP, D4-DnHP (for BBzP), D4-DCHP, D4-DBP, D4-DiBP, D4-DEHP and D4-DnOP] (AccuStandard Inc., New Haven, CT). For liquid samples that did not contain lipids, 50 g samples were extracted three times with 15 mL hexane by shaking for 30 min. The combined extracts were concentrated to 2 mL using a rotary evaporator. For solid foods, the ground samples were extracted with 20 mL acetone:hexane (1:1, vol:vol) three times, and centrifuged after shaking for 30 min. The upper organic layers were combined, concentrated to 1 mL, and transferred to 35 mL glass tubes with PTFE (Teflon) caps. After adding 30 mL hexane–saturated acetonitrile solution and 3 mL hexane, samples were shaken for 10 min and the upper layer was discarded (this was repeated three times). For cookies, which contained large amounts of lipids, after three extractions, the extract was placed in a refrigerator for half an hour and the upper layer was transferred into another tube for further concentration. The extracts were concentrated to near dryness, and then 2 mL hexane was added for further clean up by column chromatography.
A glass column packed with 7 g Florisil 60–100 mesh (Aldrich, St. Louis, MO) was used for the purification of extracts. Phthalates were eluted with 70 mL acetone:hexane (2:8, vol:vol). The final eluate was concentrated to 0.5 mL under a gentle stream of nitrogen for gas chromatography–mass spectrometry (GC-MS) analysis. For each batch of 10 samples, 3 method blanks—a spiked blank and a pair of matrix-spiked samples (spiked with 100 ng each of target phthalates) per duplicate—were processed. When the concentrations of DEHP and DBP in the 3 procedural blanks varied widely, and if the difference in concentrations among the blanks exceeded 50 ng, then all the data were discarded, and samples were reanalyzed. Mean blank values were subtracted from sample values for each batch.
Instrumental analysis. The nine phthalates were measured using a gas chromatograph (Agilent Technologies 6890N; Agilent Technologies, Santa Clara, CA) coupled with a mass spectrometer (Agilent Technologies 5973) (GC-MS) in the selective ion monitoring mode. A fused-silica capillary column (DB-5; 30 m × 0.25 mm i.d.; 0.25 mm film thickness) was used for separation. Samples (1 µL) were injected in the splitless mode. The temperatures of the injector and ion source were 280°C and 230°C, respectively. The oven temperature was programmed from 80°C (held for 1.0 min), raised to 180°C at 12°C/min (held for 1.0 min), increased to 230°C at 6°C/min, then to 270°C at 8°C/min (held for 2.0 min), and finally to 300°C at 30°C/min (held for 12 min). Ion fragments m/z 163, m/z 279, and m/z 149 were monitored for DMP, DnOP, and the other seven phthalates, and fragment m/z 206 was used for confirmation of BBzP. Deuterated internal standards for each phthalate were used for quantification. DiBP, DBP, and DEHP were present in all procedural blanks (n = 17) at mean concentrations of 0.2 (range, 0.04–0.31), 1.4 (range, 0.16–8.0), and 3.7 (range, 0.53–7.4) ng/g, respectively. These values were subtracted from sample values. The limits of detection (LODs) were 1.4, 3.7, and 1.0 ng/kg for DBP, DEHP, and DnOP, respectively, and 0.2 ng/kg for the other phthalates.
Dietary intake. Dietary intakes for adults were estimated for major groups of foods (e.g., fruits/vegetables, grain, beef) (Table 1), and intakes for infants were estimated based on infant food samples. Two different intakes were derived, one as the product of a mean food group consumption rate and a mean food group concentration, and the second as the product of a mean food group consumption rate and the median food group concentration. Food group concentrations were derived using one-half the LOD as the value for samples below the LOD, and after substituting 0 for values < LOD. Dietary intake estimates were very similar for both approaches; therefore, we report intake estimates using one-half LOD only. The food consumption rates for adults were those recommended for estimating general adult population exposures by the 2011 Exposure Factors Handbook (EFH) based on the U.S. Environmental Protection Agency (EPA) analyses of National Health and Nutrition Examination Survey (NHANES) 2003–2006 data (U.S. EPA 2011b). The “condiments” food group included pancake syrup, marinade, barbecue sauce, Italian dressing, ranch dressing, and tomato ketchup samples. A total daily consumption rate of 11.5 g/day was not provided in the EFH but was instead derived using the U.S. Department of Agriculture (USDA) Food and Nutrient Database for Dietary Studies (FNDDS 4.1; Bodner-Montville et al. 2006), which is the database associated with NHANES (Dinovi and Brookmire 2011). The food consumption rate for infants was the mean consumption rate for infants between 6 and 12 months of age from the U.S. EPA’s Child-Specific Exposure Factors Handbook (U.S. EPA 2008).
Consumption rates of each food group, in units of grams per kilogram per day, are shown in Table 1, along with the individual food products constituting each food group and the container types for the individual food samples. Of the food groups evaluated for adults, condiments had the lowest estimated consumption rate (0.2 g/kg/day) and beverages had the highest (13 g/kg/day). The estimated consumption rate for infant foods was 56 g/kg/day.
The frequency of detection of phthalates in various food types varied from zero to 100% (Table 2). Despite this, we detected at least some phthalates in every food group in this study. DEHP was detected in 74% of food samples, including all seven infant food samples, whereas DCHP was detected in only 6% of food samples (Table 2). DEHP was not detected in the two beef samples, but was found in three of four samples of pork, five of six samples of chicken, and in the hot dog sample, which was not included in any other food group. Other phthalate esters were detected at the following frequencies: DEP (57%), DiBP (55%), BBzP (54%), DMP (37%), and DBP (31%).
DEHP had the highest concentration of the phthalates tested for all food categories except beef and vegetable oils, ranging from a mean of 4 ng/mL for beverages to 300 ng/g for pork (Table 3). As noted above, DCHP was the least frequently detected phthalate, quantified in only 4 of 65 samples (one in each of four food groups), including 3 samples with concentrations ≤ 1.9 ng/g, and 1 olive oil sample with a concentration of 42.6 ng/g [see Supplemental Material, Table S1 (http://dx.doi.org/10.1289/ehp.1206367)].Median concentrations were consistently lower than mean concentrations, and for some food groups the discrepancy was large. For example, the mean value of BBzP in vegetable oil (154 ng/g, compared with the median value of 2.2 ng/g) (Table 3) was influenced by a single olive oil sample with a high concentration (459 ng/g) relative to the other oil samples (0.35 and 2.2 ng/g) (see Supplemental Material, Table S1). The olive oil sample was contained in a glass jar, whereas the canola and vegetable oil samples were contained in plastic containers. The concentration of DEHP in the olive oil sample was also high (300 ng/g). DBP was found in 5 of 9 samples of “other dairy” (dairy products other than milk, including 2 cheese samples with concentrations of 138 and 513 ng/g), and in 6 of 7 grain samples (average concentration = 16 ng/g). Other noteworthy individual samples included a ham sample with 1,158 ng/g DEHP and a bread sample with 78.8 ng/g DEP. Overall, these data indicate substantial heterogeneity in phthalate concentrations, even within food groups.
Table 4 shows a comparison of mean phthalate concentrations in food groups from our survey with concentrations previously reported in the literature. One, published by Wormuth et al. (2006), was an assessment of phthalates in Europe, Asia, and North America. They reported on a dozen studies from different countries around the world to derive food and food group–specific concentrations. These studies were published between 1995 and 2005, with half published before 2000. An earlier study by Health Canada (Page and Lacroix 1995) assessed phthalate occurrence in 260 samples of foods, 98 samples of food composites, and samples of food packaging materials. A third was conducted by the Food Standards Agency (FSA) of the United Kingdom and included analysis of 20 composite samples, 29 samples of food packaging materials, and 261 individual samples, all collected in 2005 and later (FSA 2012). Finally, Fierens et al. (2012) reported on a sampling of 400 food products in Belgium in 2009 and 2010. Concentrations found in the present study appear somewhat comparable to those reported by Wormuth et al. (2006) for fish and for values derived by averaging mean values reported by Wormuth and colleagues for groups of beverages and dairy products; however, Wormuth and colleagues’ compilation describes concentrations that are about an order of magnitude higher for meat products for DEHP and DBP. The earlier study from Health Canada (Page and Lacroix 1995) occurred in the late 1980s, so the relevance here is questionable. However, it is certainly noteworthy that it is the only major study of phthalates in food in North America that we found in the literature. BBzP was noted as being not detected in most samples–it was found only in some grain and a few dairy products. DEHP was found most often and at higher concentrations than reported in more recent studies. The one poultry sample had a high concentration of 2,600 ng/g. The detection limits of the FSA (2012) survey were comparable to detection limits achieved here, ranging from about 5 to 20 ng/g. The FSA (2012) results appear to be the most comparable to our data among all the studies shown in Table 4. DEHP was generally the phthalate with the highest concentrations measured in foods in the FSA (2012) survey, as in the present study, and nearly all DEHP concentrations reported for the FSA survey are within a factor of 5 of our data. Results for DBP also compare very favorably between the FSA data and our data, whereas the compilation of Wormuth et al. (2006) reported higher concentrations of this phthalate ester. A similar comparability is seen in the comparison results for BBzP and DiBP between our study and the U.K. study (FSA 2012), with many showing average concentrations of < 1 ng/g or being nondetectable. Generally, the lowest concentrations in food were measured from samples taken in Belgian markets (Fierens et al. 2012), with median values that were often very low or nondetectable. However, similar to the findings of other studies, DEHP was quantified most frequently and at the highest concentrations of the phthalates tested, with median concentrations of 28 ng/g in dairy products, 86 ng/g in fish, and 44.5 ng/g in various meats. However, because DEHP and DBP are the most common contaminants in laboratory solvents and reagents, care should be exercised in quantifying these two phthalate esters (Guo and Kannan 2012).
Table 5 presents the estimated daily intake by phthalate and food group. The total estimated intake based on mean concentrations (with values < LOD imputed as one-half LOD), was highest for DEHP (0.673 μg/kg/day) followed by DBP (0.184 μg/kg/day), whereas estimated total mean intakes of all other phthalates were ≤ 0.1 μg/kg/day. Estimated intakes of all phthalates were fairly comparable between adults and infants on a body weight basis, with the exception of DEHP, where the estimated intake from infant food (4.2 μg/kg/day) was more than twice that of adults. Estimated total intakes were similar when a value of zero was assigned to samples with measured concentrations of below the LOD. As expected, intakes derived using mean concentrations were higher than intakes derived using median concentrations, sometimes by over a factor of two.
Others have estimated phthalate intakes based on measured concentrations in media and consumption rates, consistent with our approach. Most have reported higher estimated intakes, in part because they included nondietary intakes, but also because concentrations measured in food surveys were often higher than measured in our food samples. Wormuth et al. (2006) estimated total phthalate intakes for Europeans using a multi-pathway approach that accounted for food and non-food pathways of exposure, including ingestion of dust, soil, and personal care products; mouthing plastic toys and other products; dermal exposure to personal care products, gloves, and other sources; and inhalation (e.g., of phthalates in indoor and outdoor air and paints). Estimated total intakes from all sources reported by Wormuth and colleagues for DEHP, DBP, BBzP, and DiBP were approximately 3.0, 4.0, 0.4, and 0.6 μg/kg/day, respectively (based on data reported in Figure 5 of Wormuth et al. 2006), compared to estimated total dietary intakes of 0.7, 0.2, 0.1, and 0.02 μg/kg/day based on our food survey. They noted that estimated exposures from all sources were higher for infants than older children and adults, approximately 10 μg/kg/day for DEP and DEHP, whereas we estimated infant dietary intakes of 4.2 μg/kg/day for DEHP and only 0.02 μg/kg/day for DEP. Wormuth et al. (2006) concluded that, for adults, ingestion of food was the dominant exposure pathway for DEHP, DiBP, and DIDP, whereas personal care products dominated for DEP exposure and inhalation pathways dominated for DMP.
In addition to including nondietary sources of exposure, previous estimates may have been higher because of different exposure patterns in other populations or because of differences in the food samples measured to determine average concentrations. Phthalate contaminants in laboratory products may also result in higher estimated concentrations in food samples, as discussed by Guo and Kannan (2012). Phthalates are present in many laboratory products, including plastic materials (e.g., pipette tips), glassware, organic solvents, and sorbents, as well as in laboratory air and dust. Organic solvents used to extract target compounds from foods may also be a major source of phthalates, especially DEHP, DBP, and DIBP. We analyzed several laboratory products for the measurement of phthalate concentrations, as reported in detail elsewhere (Guo and Kannan 2012), and devoted considerable effort to developing a reliable method for measuring phthalates in food that introduces negligible levels of contamination. Despite this, there are several limitations in our analytical method. First, despite our efforts to reduce background levels of contamination, DEHP and DBP were still found in all procedural blanks. However, data were discarded if the concentrations of DEHP and DBP in the three procedural blanks varied widely, and the samples were reanalyzed until reliable and reproducible data were obtained. Second, we applied a conservative approach of subtracting the highest blank values from the concentrations measured in food samples, which may have underestimated the actual concentrations in foods. Third, our analytical method has not been validated for all categories of foods, although it has been validated for oils and fats, which pose the greatest challenges for the analysis of trace levels of lipophilic compounds. In addition, we included labeled internal standards in all food samples analyzed, which should enhance the validity of the analytical results.
While the present study focused only on food exposures, as did our previous studies on other classes of contaminants in food (Schecter et al. 2006, 2010, 2012), the use of phthalates in many consumer products has been recognized within the scientific and regulatory community as an important issue for human exposure. The U.S. EPA’s Office of Pollution Prevention and Toxics’ Existing Chemicals Program (U.S. EPA 2011a) outlines the U.S. EPA’s activities regarding cumulative exposures to phthalates. They identify eight phthalates (DBP, DiBP, BBzP, DnHP, DEHP, DnOP, DINP, and DIDP) for which they are currently obtaining data and determining regulatory options in the context of a Phthalates Action Plan (U.S. EPA 2011c), and all but DnOP and DINP are undergoing an extensive review by the U.S. EPA’s Integrated Risk Information System (IRIS) program that will include assessments of each individual phthalate and a cumulative risk assessment. The National Academies of Science recognized the importance of cumulative exposure—generally and specifically—to phthalates when they released the report, Phthalates and Cumulative Risk Assessment—The Task Ahead, in 2008 (National Academies of Science 2008). Finally, the Consumer Product Safety Commission (CPSC) initiated a Chronic Hazard Advisory Panel on Phthalates in 2010 to study cumulative exposure and potential health effects of phthalates, with a particular emphasis on children’s exposure to phthalates in toys (CPSC 2010).
Once these U.S. EPA and CPSC efforts are completed, likely during 2013, more data will be available to estimate cumulative exposures to the phthalates measured in our survey. Until then, there are a limited number of individual benchmarks with which to compare the dietary intakes estimated in this study. Our data suggest that dietary intakes of individual phthalates are much less than currently published U.S. EPA reference dose (RfD) benchmarks, although it is important to note that RfDs pertain to all pathways of exposure. For example, the currently published RfD for DEHP on the U.S. EPA’s IRIS database is 20 μg/kg/day (U.S. EPA 2012c), whereas our estimate of total dietary intake is only 0.7 μg/kg/day. Similarly, our estimated dietary intakes of 0.2 μg/kg/day for DBP, 0.03 μg/kg/day for DEP, and 0.085 μg/kg/day for BBzP are substantially lower than corresponding published RfDs of 100 μg/kg/day (U.S. EPA 2012b), 800 μg/kg/day (U.S. EPA 2012d), and 200 μg/kg/day (U.S. EPA 2012a), respectively. To date, the U.S. EPA has not published RfDs for the other phthalates measured in this study.
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