Errata – Investigation of Microcystin Congener–Dependent Uptake into Primary Murine Neurons
Environ Health Perspect 121:a73–a73 (2013). http://dx.doi.org/10.1289/ehp.121-a73 [Online 1 March 2013]
Feurstein et al. have reported errors in their paper “Investigation of Microcystin Congener–Dependent Uptake into Primary Murine Neurons” [Environ Health Perspect 118:1370–1375 (2010)]: In “Materials and Methods” under “PP [protein phosphatase] activity after exposure to single MC congeners” (p. 1371), some of the concentrations given for enzyme solution buffer and reaction buffer represent stock concentrations instead of final concentrations.
The corrected text is as follows:
Cells were exposed to varying concentrations of single MC congeners for 48 hr and solubilized in 50 µL enzyme solution buffer [52 mM Tris-HCl (pH 7.0), 0.1 mM ethylene glycol tetraacetic acid, 1 mM dithiothreitol (DTT; in 0.01 M sodium acetate, pH 5.2), 2 mM manganese chloride (MnCl2)]. Protein concentrations were determined using the method of Bradford (1976). Subsequently, 20 µL of each sample (containing 10 µg total protein) was transferred to a 96-well plate, and an equal volume of water was added. To determine PP activity, 200 µL freshly prepared and prewarmed (37°C) substrate solution containing reaction buffer [125 mM Tris-HCl (pH 8.1), 0.4 mM MnCl2, 52 mM magnesium chloride, 1 mg/mL bovine serum albumin (BSA)], 60 mM p-nitrophenyl phosphate (pNPP; Acros Organics, Morris Plains, NJ, USA), and 2 mM DTT were added and immediately measured at 405 nm (TECAN infinite M200; TECAN, Craislheim, Germany) to determine the 0-time value.
These changes do not affect the results or conclusions of the paper.
The authors regret the errors.
The January news article “Beyond Uncertainty Factors: Protecting the Tails of the Bell Curve” [Environ Health Perspect 121:A26–A29 (2013)] incorrectly cited the 1997 book Our Stolen Future as the source of the figures and related quotation on p. A28. The figures and quotation actually came from the website http://www.ourstolenfuture.org/ and were developed by John Peterson Myers, a coauthor of the 1997 book. However, they did not appear in the book itself.
EHP regrets the error.
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EHP is proud to announce its 2013 Paper of the Year is “450K Epigenome-Wide Scan Identifies Differential DNA Methylation in Newborns Related to Maternal Smoking during Pregnancy” [Environ Health Perspect 120(10):1425–1431 (2012); doi:10.1289/ehp.1205412]. The Paper of the Year award is given to the most highly cited Research Article, Commentary, or Review Article published in the preceding 12 months.
Congratulations to authors Bonnie R. Joubert, Siri E. Håberg, Roy M. Nilsen, Xuting Wang, Stein E. Vollset, Susan K. Murphy, Zhiqing Huang, Cathrine Hoyo, Øivind Midttun, Lea A. Cupul-Uicab, Per M. Ueland, Michael C. Wu, Wenche Nystad, Douglas A. Bell, Shyamal D. Peddada, and Stephanie J. London.
Children’s Health Collection 2013 is now available for browsing and downloading. The collection includes everything we've published on children's health from October 2012 through September 2013—you'll find abstracts of peer-reviewed research articles, reviews, and commentaries, plus quick links to relevant news articles, editorials, and podcasts.
Environment and Health—
Bridging South, North, East and West
Basel, Switzerland 19–23 August 2013
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