Errata – Investigation of Microcystin Congener–Dependent Uptake into Primary Murine Neurons
Environ Health Perspect 121:a73–a73 (2013). http://dx.doi.org/10.1289/ehp.121-a73 [Online 1 March 2013]
Feurstein et al. have reported errors in their paper “Investigation of Microcystin Congener–Dependent Uptake into Primary Murine Neurons” [Environ Health Perspect 118:1370–1375 (2010)]: In “Materials and Methods” under “PP [protein phosphatase] activity after exposure to single MC congeners” (p. 1371), some of the concentrations given for enzyme solution buffer and reaction buffer represent stock concentrations instead of final concentrations.
The corrected text is as follows:
Cells were exposed to varying concentrations of single MC congeners for 48 hr and solubilized in 50 µL enzyme solution buffer [52 mM Tris-HCl (pH 7.0), 0.1 mM ethylene glycol tetraacetic acid, 1 mM dithiothreitol (DTT; in 0.01 M sodium acetate, pH 5.2), 2 mM manganese chloride (MnCl2)]. Protein concentrations were determined using the method of Bradford (1976). Subsequently, 20 µL of each sample (containing 10 µg total protein) was transferred to a 96-well plate, and an equal volume of water was added. To determine PP activity, 200 µL freshly prepared and prewarmed (37°C) substrate solution containing reaction buffer [125 mM Tris-HCl (pH 8.1), 0.4 mM MnCl2, 52 mM magnesium chloride, 1 mg/mL bovine serum albumin (BSA)], 60 mM p-nitrophenyl phosphate (pNPP; Acros Organics, Morris Plains, NJ, USA), and 2 mM DTT were added and immediately measured at 405 nm (TECAN infinite M200; TECAN, Craislheim, Germany) to determine the 0-time value.
These changes do not affect the results or conclusions of the paper.
The authors regret the errors.
The January news article “Beyond Uncertainty Factors: Protecting the Tails of the Bell Curve” [Environ Health Perspect 121:A26–A29 (2013)] incorrectly cited the 1997 book Our Stolen Future as the source of the figures and related quotation on p. A28. The figures and quotation actually came from the website http://www.ourstolenfuture.org/ and were developed by John Peterson Myers, a coauthor of the 1997 book. However, they did not appear in the book itself.
EHP regrets the error.
CEHN December 2014 Article of the Month
“The Navigation Guide—Evidence-Based Medicine Meets Environmental Health: Integration of Animal and Human Evidence for PFOA Effects on Fetal Growth” (Environ Health Perspect; DOI:10.1289/ehp.1307923) has been selected by the Children’s Environmental Health Network (CEHN) as its December 2014 Article of the Month. These CEHN summaries discuss the potential policy implications of current children’s environmental health research.
Introducing Children’s Health Collection 2014
EHP’s fifth annual Children’s Health Collection is now available. The collection comprises abstracts of all relevant articles published in EHP from October 2013 through September 2014: peer-reviewed research articles, news features, Science Selections, and editorials.
ISEE 2014 Abstracts Now Available
EHP is pleased to present the abstracts for the 26th annual conference of the International Society for Environmental Epidemiology (ISEE), From Local to Global: Advancing Science for Policy in Environmental Health, held 24–28 August 2014 in Seattle, Washington.
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Recent Advance Publications
Developmental Exposure to a Commercial PBDE Mixture: Effects on Protein Networks in the Cerebellum and Hippocampus of Rats
Attention Deficit/Hyperactivity Disorder and Childhood Autism in Association with Prenatal Exposure to Perfluoroalkyl Substances: A Nested Case–Control Study in the Danish National Birth Cohort
Autism Spectrum Disorder and Particulate Matter Air Pollution before, during, and after Pregnancy: A Nested Case–Control Analysis within the Nurses’ Health Study II Cohort
Comparative Effects of Di(n-Butyl) Phthalate Exposure on Fetal Germ Cell Development in the Rat and in Human Fetal Testis Xenografts
Interaction Effects of Temperature and Ozone on Lung Function and Markers of Systemic Inflammation, Coagulation, and Fibrinolysis: A Crossover Study of Healthy Young Volunteers