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Environ Health Perspect; DOI:10.1289/EHP54

Combined Effects of in Utero and Adolescent Tobacco Smoke Exposure on Lung Function in C57Bl/6J Mice

David Drummond,*1 Mélissa Baravalle-Einaudi,*1 Guillaume Lezmi,1 Shamila Vibhushan,1 Marie-Laure Franco-Montoya,1 Alice Hadchouel,1,2,3 Jorge Boczkowski,1 and Christophe Delacourt1,2,3
Author Affiliations open
1INSERM, U955, IMRB, Equipe 04, Créteil, France; 2Pneumologie Pédiatrique, Necker, AP-HP, France; Centre de Référence des Maladies Respiratoires Rares, Paris, France; 3Université Paris-Descartes, Paris, France

*contributed equally to this work

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  • Background: Foetal determinants of airway function, such as in utero exposure to maternal cigarette smoke (CS), may create a predisposition to adult airflow obstruction and COPD in adulthood. It has been suggested that active smoking in adolescence and pre-existing airflow obstruction have synergistic deleterious effects.

    Objective: We used a mouse model to investigate whether there is a synergistic effect of exposure to CS in utero and during adolescence on lung function.

    Methods: Female C57Bl/6J mice were exposed to CS or to filtered room air during pregnancy. Exposure to CS began two weeks before mating, and continued until delivery. After birth, the pups were not exposed to CS until day (D)21. Between D21 and D49, corresponding to “adolescence”, litters were randomized for an additional 4 weeks of exposure to CS. Lung morphometry, lung mechanics, and the expression of genes involved in senescence were evaluated in different subsets of mice on D21 and D49.

    Results: In utero exposure to CS induced significant lung function impairment by D21. CS exposure between D21 and D49 induced significant functional impairment only in mice exposed to CS prenatally. On D49, no difference was observed between subgroups in terms of lung p53, p16, p21, and Bax mRNA levels.

    Conclusions: Our findings suggest that prenatal and adolescent CS exposure have a synergistic effect on lung function in mice. The combined effect did not appear to be a consequence of early pulmonary senescence.

  • This EHP Advance Publication article has been peer-reviewed, revised, and accepted for publication. EHP Advance Publication articles are completely citable using the DOI number assigned to the article. This document will be replaced with the copyedited and formatted version as soon as it is available. Through the DOI number used in the citation, you will be able to access this document at each stage of the publication process.

    Citation: Drummond D, Baravalle-Einaudi M, Lezmi G, Vibhushan S, Franco-Montoya ML, Hadchouel A, Boczkowski J, Delacourt C. Combined Effects of in Utero and Adolescent Tobacco Smoke Exposure on Lung Function in C57Bl/6J Mice. Environ Health Perspect; http://dx.doi.org/10.1289/EHP54

    Received: 2 March 2016
    Revised: 10 October 2016
    Accepted: 13 October 2016
    Published: 4 November 2016

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