News | Science Selections August 2008 | Volume 116 | Issue 8
Alternative Mechanism for PFOA?: Trout Studies Shed Light on Liver Effects
Citation: Hood E. 2008. Alternative Mechanism for PFOA? Trout Studies Shed Light on Liver Effects. Environ Health Perspect 116:A351; http://dx.doi.org/10.1289/ehp.116-a351b
Published: 1 August 2008
Perfluorooctanoic acid (PFOA), used to make a class of industrial chemicals that are widely used in products such as textile coatings and flame retardants, is known to be a potent hepatocarcinogen in rodents. Until now the only mechanism of action for PFOA identified in rodent studies has been peroxisome proliferation, a well-characterized form of oxidative stress. Humans are reportedly insensitive to peroxisome proliferation; however, concerns remain that PFOA may cause adverse effects in people as well as in laboratory animals. Using rainbow trout as a model for chemically induced liver cancer in humans, a team of researchers suggest a new mechanism for the carcinogenicity of PFOA that does not involve peroxisome proliferation [EHP 116:1047–1055; Tilton et al.].
The investigators compared the hepatocarcinogenic potential of PFOA against the structurally diverse peroxisome proliferators cloribrate (CLOF) and dehydroepiandrosterone (DHEA), identifying mechanisms of carcinogenesis from hepatic gene expression profiles phenotypically anchored to tumor outcome. Trout were fed PFOA in the diet for 30 weeks for tumor analysis. The investigators subsequently examined gene expression by cDNA array in animals fed PFOA, DHEA, CHOF, or 17β-estradiol (E2; a known tumor promoter) in the diet for 14 days.
The study showed that PFOA and DHEA treatments significantly increased liver tumor incidence and multiplicity; CLOF showed no effect. However, carcinogenesis was independent of peroxisome proliferation as measured by the lack of peroxisomal β-oxidation and catalase activity. On the contrary, both PFOA and DHEA resulted in estrogenic gene signatures closely resembling that of E2. CLOF, however, regulated no genes in common with E2.
Although the current study did not identify a threshold for the estrogenic effect of PFOA, the results indicate that PFOA is weakly estrogenic in trout and that its association with trout liver cancer may be related to disruptions in estrogenic signaling. More studies are needed to assess the potential for PFOA-mediated carcinogenesis in other species that are insensitive to peroxisome proliferation. Considering the mechanism identified in this study, the consequences of hormone-related effects by PFOA should be evaluated in other tissues, models, and sensitive life stages.
EHP is pleased to announce that it is now operating under a continuous publication workflow! As indicated in a previous announcement, continuous publication allows EHP to post new content online throughout the month, as each paper becomes ready for an issue. This gets content out to our readers much more quickly than the old issue-based model, and unlike our previous Advance Publication model, these are final, edited articles. (more…)
EHP is pleased to announce that Prenatal Exposure to Glycol Ethers and Neurocognitive Abilities in 6-Year-Old Children: The PELAGIE Cohort Study, published in EHP on 14 October 2016, has been selected by the Children’s Environmental Health Network (CEHN) as its May 2017 Article of the Month. CEHN Article of the Month summaries discuss the potential policy implications of current children’s environmental health research. The CEHN summary can be viewed here.