PM2.5 Concentrations in a Cannabis Store with On-Site Consumption

Introduction Recently, California and other states have legalized the use of cannabis in licensed stores (subject to state-specific limitations), giving people who cannot consume cannabis in their homes a safe and legal place to consume it. However, on-site consumption may expose customers and workers to particulate air pollution. Consumption methods that use temperatures below combustion to aerosolize cannabis are a way to reduce exposure to toxicants (Gieringer et al. 2004). In vaporization of cannabis flower, an aerosol is formed by passing heated air through finely ground, dried flower. Cannabis concentrates can be consumed by dabbing, where a small amount of concentrate is applied to a heated surface to create an aerosol. Like smoking, vaporizing and dabbing create aerosols that contain particles ≤2:5 lm in aerodynamic diameter (PM2:5) (Jaques et al. 2018) that can penetrate deep into the lung. To assess the effects of on-site consumption of cannabis on PM2:5 concentrations, we measured PM2:5 in the retail and consumption space of a cannabis store (a dispensary), where smoking was banned, but vaporizing and dabbing were permitted.


Introduction
Recently, California and other states have legalized the use of cannabis in licensed stores (subject to state-specific limitations), giving people who cannot consume cannabis in their homes a safe and legal place to consume it. However, on-site consumption may expose customers and workers to particulate air pollution. Consumption methods that use temperatures below combustion to aerosolize cannabis are a way to reduce exposure to toxicants (Gieringer et al. 2004). In vaporization of cannabis flower, an aerosol is formed by passing heated air through finely ground, dried flower. Cannabis concentrates can be consumed by dabbing, where a small amount of concentrate is applied to a heated surface to create an aerosol. Like smoking, vaporizing and dabbing create aerosols that contain particles ≤2:5 lm in aerodynamic diameter (PM 2:5 ) (Jaques et al. 2018) that can penetrate deep into the lung. To assess the effects of on-site consumption of cannabis on PM 2:5 concentrations, we measured PM 2:5 in the retail and consumption space of a cannabis store (a dispensary), where smoking was banned, but vaporizing and dabbing were permitted.

Methods
PM 2:5 concentrations were measured continuously, using two, colocated laser photometers (model AM510; TSI Inc.), placed 80-100 cm above the floor, for 5 wk in 2019. Room occupancy was not monitored. In Wk 1, the instruments were located 30-122 cm from the sources (vaporizers and dab rigs). During Wk 2 and Wk 3-5, they were 6-9 and 2-4 m from the nearest sources, respectively. Photometers were operated with impactors to exclude particles >2:5 lm in diameter. The photometers were zeroed once a day and calibrated gravimetrically using a controlled cigarette smoke generation system (Schick et al. 2012) before and after each experiment. Gravimetric data from 20 cigarette smoke experiments, when plotted against the matching photometric data and forced through zero, yielded a calibration factor of 0.31 (R 2 = 0:84), which was applied to the dispensary photometric data. Cannabis PM 2:5 samples were also collected in the dispensary on filters (Emfab; Pall Corporation) for 1 wk in December 2019, and a preliminary photometer calibration factor was calculated as above. PM 2:5 concentrations in outdoor air were estimated using data from a U.S. Environmental Protection Agency monitoring station located 2:5 km (1:5 mi) from the dispensary in an area with similar ambient pollution sources.

Results
The retail and consumption space was a single room of ∼ 400 m 3 . Cannabis consumption occurred at three tables in one corner of the room, with sales counters located in the opposite corner. The room was served by the building heating, ventilation, and air conditioning (HVAC) system and by four window air conditioners that did not admit fresh air. The air conditioners had dust filters, and we were unable to examine filtration in the building HVAC system. The dispensary provided electrically heated cannabis flower vaporizers and dab rigs for use. Smoking (combustion) of cannabis and tobacco were not permitted.
We monitored PM 2:5 in the dispensary for 38 d and 16 h. During business hours, the average PM 2:5 concentration was 84 lg=m 3 , with a standard deviation of ± 124 lg=m 3 (Figure 1), an interquartile range (IQR) of 16-111 lg=m 3 , and a median of 47 lg=m 3 . When the business was closed, the average PM 2:5 concentration was 3 ± 7 lg=m 3 , the IQR was 1-4 lg=m 3 , and the median was 2 lg=m 3 . When examined in 2-h intervals, the median PM 2:5 concentration was highest between 1700 and 1900 hours, at 76 lg=m 3 (Figure 2). The average PM 2:5 concentration outdoors was 6 ± 4 lg=m 3 during business hours and 6 ± 5 lg=m 3 when the business was closed. The dispensary gravimetric data yielded a photometer calibration factor of 0.57 (R 2 = 0:43).

Discussion
Our data show a clear association between the consumption of cannabis and elevated PM 2:5 concentrations in the dispensary. The average PM 2:5 concentration when the business was open was 28 times higher than when the business was closed, the median concentration was 23.5 times higher, and peak daily particle concentrations corresponded with the busiest hours. The PM 2:5 concentrations in this cannabis dispensary are similar to those observed in indoor spaces where smoking is permitted (California Air Resources Board 2005). These findings are some of the first field measurements of PM 2:5 emissions from cannabis flower vaporizers and dabbing of cannabis concentrates. In a space with similar ventilation and consumption activity, it is likely that dabbing and vaporizing would create lower PM 2:5 concentrations than smoking, because smoking decomposes the cannabis more completely, creating more sidestream smoke.

Limitations
Most of our data are from TSI Sidepak laser photometers, which are factory-calibrated to National Institute of Standards and Technology Standard A1 test dust (ISO 12103-1). To deliver accurate measurements of any other aerosol, a specific calibration factor would be required. As of this writing, there are no published calibration factors for aerosols created by vaporizing cannabis flower or dabbing cannabis concentrates and little is known of their properties. The gravimetric data from the dispensary yielded a calibration factor of 0.57, but variation was high (R 2 = 0:41) because there were only seven day-long samples. We therefore used the well-validated calibration factor for secondhand cigarette smoke (0.31) (Hyland et al. 2008) to adjust our data. This calibration factor is unlikely to yield inflated values, and if the true calibration factor is higher, that does not affect our finding that on-site consumption was associated with strong and consistent increases in PM 2:5 .
Note to readers with disabilities: EHP strives to ensure that all journal content is accessible to all readers. However, some figures and Supplemental Material published in EHP articles may not conform to 508 standards due to the complexity of the information being presented. If you need assistance accessing journal content, please contact ehponline@niehs.nih.gov. Our staff will work with you to assess and meet your accessibility needs within 3 working days.

Conclusion
Our data demonstrate that consumption of cannabis products indoors increased PM 2:5 concentrations. Psychoactive effects through passive exposure are unlikely (Herrmann et al. 2015). However, exposure to PM 2:5 can cause changes in cardiovascular function that increase the risk of myocardial infarction and death (Brook et al. 2010). In healthy nonsmokers, even 30 min of exposure to cigarette smoke, at concentrations of <200 lg=m 3 PM 2:5 , decreased endothelial function, a well-validated predictor of increased risk of cardiovascular disease (Yeboah et al. 2009;Frey et al. 2012). It is possible that the aerosols from vaporizers and dabbing are less toxic than standard combustion aerosols. However, even brief increases in ambient PM 2:5 from mixed sources are associated with increases in myocardial infarction and total mortality (Brook et al. 2010) and these effects are detectable even at PM 2:5 increases of 10 lg=m 3 (Di et al. 2017). It is likely that the PM 2:5 concentrations we observed are high enough to cause health problems for some individuals. Further research on the toxicity of cannabis smoke and vaporizer and dabbing aerosols is necessary. . Every morning the photometer data was downloaded and the instruments were zeroed and left logging for the next 24 h. The photometers logged data every 15 s. The photometers were operated with PM 2:5 impactors to exclude larger aerosol particles and the impactors were cleaned every 72 h. The photometer air flow was set to 1.7 L/min and calibrated once a week with a soap bubble spirometer (Gilibrator-1; Sensidyne, LP). Note: PM 2:5 , particulate matter ≤2:5 lm in aerodynamic diameter. Whiskers are 10th and 90th percentiles, and circles are 5th and 95th percentiles. Note: PM 2:5 , particulate matter ≤2:5 lm in aerodynamic diameter.