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The APOBEC3 gene family plays a role in innate cellular immunity inhibiting retroviral infection, hepatitis B virus propagation, and the retrotransposition of endogenous elements. We present a detailed sequence and population genetic analysis of a 29.5-kb common human deletion polymorphism that removes the APOBEC3B gene. We developed a PCR-based genotyping assay, characterized 1,277 human diversity samples, and found that the frequency of the deletion allele varies significantly among major continental groups (global F ST ¼ 0.2843). The deletion is rare in Africans and Europeans (frequency of 0.9% and 6%), more common in East Asians and Amerindians (36.9% and 57.7%), and almost fixed in Oceanic populations (92.9%). Despite a worldwide frequency of 22.5%, analysis of data from the International HapMap Project reveals that no single existing tag single nucleotide polymorphism may serve as a surrogate for the deletion variant, emphasizing that without careful analysis its phenotypic impact may be overlooked in association studies. Application of haplotype-based tests for selection revealed potential pitfalls in the direct application of existing methods to the analysis of genomic structural variation. These data emphasize the importance of directly genotyping structural variation in association studies and of accurately resolving variant breakpoints before proceeding with more detailed population-genetic analysis.


quence and po
ulation genetic analysis of a 29.5-kb common human deletion polymorphism that removes the APOBEC3B gene.We developed a PCR-based genotyping assay, characterized 1,277 human diversity samples, and found that the frequency of the deletion allele varies significantly among major continental groups (global F ST ¼ 0.2843).The deletion is rare in Africans and Europeans (frequency of 0.9% and 6%), more common in East Asians and Amerindians (36.9% and 57.7%), and almost fixed in Oceanic populations (92.9%).Despite a worldwide frequency of 22.5%, analysis of data from the International HapMap Project reveals that no single existing tag single nucleotide polymorphism may serve as a surrogate for the deletion variant, emphasizing that without careful analysis its phenotypic impact may be overlooked in association studies.Application of haplotype-based tests for selection revealed potential pitfalls in the direct application of existing methods to the analysis of genomic structural variation.
hese data emphasize the importance of directly genotyping structural variation in association studies and of accurately resolving variant breakpoints before proceeding with more detailed population-genetic analysis.

Introduction

The APOBEC3 family is known to play a role in innate cellular immunity against retroviral infection.The gene family has undergone an expansion in primates, increasing from a single copy in rodents to at least seven copies in humans [1][2][3].Among primates, the APOBEC3 family has been subjected to strong and continuing selective pressures at the amino acid level [3,4].APOBEC3 proteins defend against retroviruses by deaminating cytosine residues to uracil, resulting in hypermutation and degradation of the viral genome.Members of this gene family contain either one (APOBEC3A and APOBEC3C) or two (APOBEC3B, APOBEC3F, and APOBEC3G) conserved cytosine deamination domains [1,2].In addition to their role in innate retroviral immunity, some APOBEC3 genes appear to inhibit hepatitis B virus infection [5][6][7][8] and the retrotransposition of endogenous elements [9][10][11][12].It is thought that at least part of this activity occurs through a deamination-independent mechanism [12].

Several recent studies have brought increased attention to classes of genomic variation such as deletions, inversions, and copy-number polymorphisms [13][14][15][16][17][18][19][20].It is thought that these variations contribute substantially to inter-individual genomic, and perhaps, phenotypic variation, but the structure and population characteristics of these variants remain largely unexplored.A deletion in the APOBEC3 gene cluster was recently identified using two different approaches.The deletion was first discovered by mapping end-sequence pairs from a human fosmid library against the human genome reference sequence assembly [16].A cluster of discordant fosmid clones whose end-sequences mapped further apart than the expected fosmid i

redicted a deletion of ;30 kb near the APOBEC3B ge
e.Later, a second approach confirmed the deletion based on an interrogation of a dense single nucleotide polymorphism (SNP) marker map generated as part of the International HapMap Project [17].This method discovered deletions by identifying clusters of SNPs that showed apparent non-Mendelian inheritance, deviations from Hardy-Weinberg equilibrium, or evidence of null genotypes.Nevertheless, this variant was not detected in a recent genome-wide screen of structural variation in the HapMap populations using BAC or SNP-based microarrays [20].The availability of a fosmid clone that captured the deletion event allowed us to sequence the structural variant in its entirety and confirm its presence.Precise sequence definition of the deletion enabled the design of specific genotyping assays across the deletion breakpoints.We present here a sequence-based analysis of this deletion polymorphism, a worldwide population survey of the deletion frequency (1,277 DNA samples), and an analysis of the surrounding haplotype structure.The results suggest this is a functionally important structural variant that is stratified in the human population.


Results


Sequence-Based Resolution of Deletion Breakpoints

We sequ

ced the entire inse
t of one of the fosmid clones whose end-sequence pairs had initially identified the structural variant.Alignment of this sequence with the sequence from the finishing human genome sequence assembly (hg17) confirmed the presence of a deletion overlapping the APOBEC3A and APOBEC3B transcripts (Figure 1).Consistent with non-allelic homologous recombination as the likely mechanism of origin, the deletion breakpoints mapped to two highly identical tracts of sequence: 350 bp in length, 100% sequence identity.In the deleted configuration, a single copy of this sequence exists and the 29.5 kb of sequence between them is removed (position 37,683,131-137,712,716 on Chromosome 22 of hg17).The deletion removes the genomic sequence between the fifth exon of APOBEC3A and the eighth exon of APOBEC3B, leading to a predicted fulllength functional hybrid transcript with a predicted amino acid composition identical to APOBEC3A.Thus, individuals possessing this structural variant would lack at least one copy of the unique coding portion of APOBEC3B.Interestingly, the predicted transcript would contain the 39 UTR from APOBEC3B, but be subject to APOBEC3A upstream regulatory sign

s.


Deletion
requency

The availability of the complete sequence of the deletion breakpoints allowed us to design PCR breakpoint assay

which distingui
hed insertion and deletion alleles (Figure 1 and Materials and Methods).We genotyped 1,007 individuals from 51 populations included in the Centre d'Etude du Polymorphisme Humain (CEPH) Human Genome Diversity Panel (HGDP) and found that the deletion frequency was highly variable (Figure 2).The deletion is rare in African and European populations (frequency of 0.9% and 6%, respectively), more common in East Asian and American populations (36.9% and 57.7%), and almost fixed in Oceanic populations (92.9%;Tables S1-S3).As a control against potential SNPs under the PCR primer binding sites leading to an overestimate of the frequency of homozygotes, we reanalyzed all 127 samples initially scored as deletion homozygotes with a second PCR assay targeted to the insertion allele (see Materials and Methods).We reclassified 25 samples (19.6%) as hemizygous (Table 1).In order to rule out further large-scale genotyping error, we calculated estimates of Hardy-Weinberg equilibrium for each population.No significant deviations were observed.


F ST Analysis

As a measure of population differentiation, we c lculated an overall F ST value of 0.2843 for the APOBEC3B deletion in


Author Summary

Several recent studies have demonstrated that deletions, duplications, and inversions contribute a substantial fraction of the total amount of variation present in the human genome.In this study, we provide a comprehensive population-genetic analysis of a single deletion previously identified by comparing the genome of a single individual against the human genome reference sequence.Complete genomic sequence spanning the deleted region was obtained, allowing us to define the deletion breakpoints and develop a direct genotyping assay.Analysis showed that the deletion removes a member of a gene family involved in the innate immune response against viral pathogens.We genotyped samples from a human diversity panel and found drastic differences in the frequency of the deletion around the world.Using data from the HapMap project and the application of existing anal sis techniques, we illustrate the importance of directly genotyping this type of variation and of clearly defining its boundaries.Without this level of detail the potential functional importance of such variation may be missed.

the HGDP.Large F ST values are consistent with either geographically restricted selection (local adaptation) or demographic history (i.e., population bottlenecks and founder effects).To distinguish between these two possibilities, we calculated an empirical F ST distribution using 2,540 autosomal SNPs and 207 small indels genotyped in individuals from the same 51 popul

ions [21,22].Of the 2,747 loci, 52 had an F ST
alue greater than that obtained for the APOBEC3B deletion, placing this deletion within the top 2% of the empirical distribution.Estimates of F ST may be sensitive to allele frequency, so we repeated this comparison by only considering the 635 loci that had a global minor allele frequency between 0.17 and 0.27.A single SNP (rs2250341, located in an intron of the PCP4 gene) of this subset had an F ST value greater than the APOBEC3B deletion, placing the deletion in the top 0.16% of the frequency-matched empirical distribution.

A striking feature of the deletion is the clinal increase in frequency as one moves eastward away from Africa (Figure S1).In order to further delineate this pattern, we repeated this analysis using pairwise F ST estimates between all possible combinations of the 51 populations (Figure S2).The analysis differentiates Oceanic, Amerindian, and some East Asian populations from other human populations based on the frequency of the deletion variant in comparison to other SNP and indel loci in the same populations.This suggests that this pattern is not solely the result of demographic history.


Linkage Disequilibrium and Haplotype Structure

We genotyped the individuals included in the HapMap project: consisting of samples from the Yoruba people of the Ibidan Peninsula in Nigeria (referred to as YRI), the CEPH project in Utah (CEU), the Han Chinese population of Beijing (CHB), and individuals of Japanese ancestry from the Tokyo area (JPT); and searched for evidence of linkage disequilibrium (LD) between the A OBEC3B deletion and flanking HapMap Phase I SNPs (Tables S4-S5).In contrast to other deletion polymorphisms [17,19], we found no single SNP to be in strong LD (r 2 greater than 0.8) with the deletion variant (Figure 3).In the Yoruba sample there was one rare SNP with an r 2 value of 0.663.This SNP, rs733107, has a minor allele frequency of 0.025 with two of the three Yoruba deletion  S3) are overlaid on a map of the world.The deletion (black area o pie chart) and insertion frequency (white) are depicted.doi:10.1371/journal.pgen.0030063.g002chromosomes carrying the minor allele.Interestingly, we also found that no two-marker combination had an r 2 value greater than 0.8 (the maximum values are 0.254 for CEU, 0.499 for JPT and CHB, and 0.661 for YRI).However, it remains possible that more sophisticated multi-marker approaches will be able to successfully tag the deletion variant using existing SNPs.Unlike other regions of the human genome, such as those enriched for complex segmental duplications, the SNP density in this region (approximately one SNP every 2

kb) is not significantly reduce
.Since the deletion frequency shows drastic differences among the HapMap populations, the absence of a suitable single marker tag is likely a consequence of a bias in the ascertainment of SNPs typed in the HapMap panel.

Although no single SNP can act as a reliable proxy for the variant, we noticed that the deletion appears to occur on a common haplotype.Treating the deletion locus as a bi-allelic variant, we constructed phased haplotypes using 21 HapMap Phase II SNPs genotyped in all populations and located within 25 kb of the deletion boundaries and performed a haplotype network analysis (Figures 4 and S3; Tables S6 and  S7) [23] (http://fluxus-engineering.com).

We identified 49 distinct haplotypes over this region.Overall, 91% of the deletion events (YRI ¼ 3/3, CEU ¼ 8/8, and 61/68 of the JPT and CHB) lie on a single common haplotype (haplotype 28) which differs from haplotype 31 only by the presence of the de

tion varian
.Two additional haplotypes are observed only for JPT and CHB deletion chromosomes: haplotype 34 (n ¼ 4 chromosomes) and haplotype 29 (n ¼ 3 chromosomes) differ from haplotype 28 by a single nucleotide difference.The former may represent an independent occurrence of the deletion on haplotype 39 or a recombination event between haplotypes 39 and 29.


Extended Haplotype Homozygosity

In order to further investigate the unusual shared haplotype structure and potential signatures of selection, we assessed the deletion haplotype for evidence of extended homozygosity [24,25].We calculated extended haplotype homozygosity (EHH), the relative extended haplotype homozygosity (REHH), and the extended haplotype length (EHL) for both the deletion and insertion alleles (Figures 5 and S4; Tables 1 and 2).Without correcting for the decreased size of the deletion haplotype, a potentially strong signal of local adaptation indicated by a high frequency extended haplotype for the deletion allele in Asia is observed (Figure 5B and 5C; Table 3).Accounting for the physical reduction in chromosome size due to the deletion, however, largely eliminates this signal in the Asian population (Table 2).Nevertheless, the haplotype analysis does suggest weak signals of selection, particularly in the Yoruba population.


Discussion

There are three important results of this examination of the APOBEC3B deletion.First, the deletion occurs between two asymmetric gene structures (APOBEC3B and 3A) and produces a hybrid transcript whose putative coding sequence maintains its frame.Despite the act that the recombination event occurs between coding exons, the amino acid sequence of the hybrid gene is identical to APOBEC3A with the net effect being complete loss of APOBEC3B and potential altered regulation of APOBEC3A due to juxtaposition of novel 39 regulatory sequences.Second, the deletion variant shows dramatic population stratification with significantly elevated F ST values observed for Eastern Asian, Oceanic, and Amerindian populations.The magnitude of the F ST values compared to a set of other genome-wide loci from the same populations suggest that these observed frequency differences are not due to demographic history alone.Third, we observe that no tag-SNP currently exists for this deletion.A sophisticated, multi-marker tagging approach may successfully tag this allele, but this approach is compli ated by the observation that the major deletion haplotype (haplotype 28) is identical to another haplotype (haplotype 31), except for the presence of the insertion allele.Thus, despite the fact that nearly 40% of the world's population carries at least one copy of this deletion, a suitable SNP surrogate does not yet exist.In light of its abundance, it is noteworthy that this variant was not detected in a recent genome-wide screen of copy-number variation in the human genome [20].

These data emphasize that the p enotypic impact of this and potentially other structural variants may be overlooked in association studies unless the structural variant is directly genotyped.We also observed that potentially misleading results can be obtained from the direct application of existing haplotype-based methods for detecting selection to structural variants.In our study, adjusting for the length of the haplotype reduced the significance of signals of selection using EHL methods (Figure 5).This highlights the importance of not only directly genotyping this type of variation, but of also clearly resolving the breakpoints of the event.If genomewide screens for selection based on EHL are performed without controlling for changes in the length of the genome sequence, artifactual signals may be observed.

Our observations of the deletion architecture, the population frequency patterns, and the haplotype structure indicate that variation at this locus may be important.Analysis also revealed a weak, suggestive signal of selection for the deletion.Since it appears that the deletion occurred once and has since spread into other populations, a complet understanding of the history of this locus would require detailed knowledge of the different selective regimes and demographic histories unique to these populations.Until a more comprehensive population genetic theory is developed and a phenotypic consequence of the deletion event is demonstrated, one must be cautious about placing too much emphasis on a potential selective advantage for the deletion.Nonetheless, several possible scenarios may account for the patterns observed at this locus.First, it is possible that a 29.5-kb deletion of a gene may have altered the properties of genetic recombination on this specific haplotype.Longrange haplotype tests are highly sensitive to variance in recombination rates among different haplotypes [25].Suppressed recombination could, in principle, retard LD decay resulting in an underestimate of the allele's age leading to erroneous signals of recent selection.If this were the case, one would expect a striking correspondence between longrange haplotype-based signatures of selection [26] and the more than 1,000 deletion polymorphisms that have now been documented for the human genome [13][14][15][16][17][18][19][20].Such a correspondence has not yet been observed, although few structural variants have been studied at this level of detail.

In a second scenario, the deletion may simply be a genetic marker for another selective event that has occurred on this specific haplotype (haplotype 28).In this model, the deletion event is a genetic hitchhiker as opposed to the causative allele.The APOBEC3 gene cluster has been subject to positive selection at many different time points during human and primate evolution [3,4,26].Thus, a partial sweep of the deletion variant could occur if individuals carrying this specific haplotype had greater resistance to specific pathogens, perhaps as a result of amino acid changes in adjacent members of the APOBEC gene family.In such a scenario, however, the fitness advantage would have to outweigh loss of the APOBEC3B gene and the potential regulatory changes of APOBEC3A incurred by the disruption to the surrounding genomic region.A similar scenario has been put forward for rearrangements associated with the alpha-globin gene family.In this case, recurrent deletions of Hba1 and Hba2 associated with alpha-thalassemia have risen to high frequency in Mediterranean and Pacific rim populations [27].Data from Papua New Guinea suggest that homozygous and hetero-   zygous genotypes confer protection against malaria and other infectious disease [28].A neutral deletion seems unlikely in light of the conservation of this gene in humans and other great ape species ( [29] and J. M. Kidd, unpublished data).Moreover, APOBEC3B has recently been shown to inhibit replication of the hepatitis B virus, an observation that may warrant further study in

ight of the frequency
f this deletion [8,30].In a third scenario, the increased frequency of the deletion could represent a shift in the balance of selective forces impacting this locus.There may be a significant cost associated with the maintenance of active cytidine deaminases due to their mutagenic potential [3,31,32].It has been shown that APOBEC3B protein is present in the nucleus of cells where it may act to repress retrotransposition in early stages of development and in the germ line [8,9].When t e threat posed by both endogenous and exogenous viral activity is high, the protective properties offered by APOBEC3B may outweigh the risks associated with its own activity.When rates of endogenous viral activity are low and when changes in environment reduce the presence of exogenous virus activity, the detrimental effects associated with APOBEC3B may outweigh its benefit, resulting in strong selective pressure in favor of the deletion.Similar arguments have recently been proposed to account for the presence of an impaired allele of the retroviral defense gene TRIM5a and is consistent with evidence of increased retroviral activity in African but not Asian apes [33,34].When it comes to innate immune system genes such as APOBEC3B, less truly may be more [35].


Materials and Methods

Sequencing and sequence analysis.A shotgun sequence assembly of the fosmid insert containing the putative deletion was generated as previously described [16].Prior to sequencing, a fingerprint map with four independent restriction enzymes (EcoRI, HindIII, BglII, and NsiI) confirmed the ;30-kb deletion [36].Deletion breakpoints were identified by comparison of the fosmid insert sequence against the human genome reference sequence assembly (hg17) using ClustalW and BLASTN [37,38].

PCR genotyping assay.We designed PCR breakpoint assays to distinguish the insertion and deletion alleles based on the following oligonucleotide sequences: Deletion_F: TAGGTGCCACCCCGAT; Delet on_R: TTGAGCATAATCTTACTCTTGTAC; Insertion1_F: TTGGTGCTGCCCCCTC; Insertion1_R: TAGAGACTGAGGCCCAT; and Insertion2_F: TGTCCCTTTTCAGAGTTTGAGTA; Inser-tion2_R: TGGAGCCAATTAATCACTTCAT. Deletion primers are specific to the deletion sequence configuration and generate a 700-bp PCR product upon amplification.Insertion1 and Insertion2 primers amplify only the insertion configuration and produce 490-and 705bp products, respectively.Insertion and deletion PCR assays were performed separately, the products pooled, and visualized on a standard 1.5% agarose gel.PCR was performed in 17-ll reactions composed of 0.85 ll o a 10-lM dilution of the forward primer, 0.85 ll of a 10-lM dilution of the reverse primer, 8.5 ll of Qiagen (http:// www1.qiagen.com)PCR mastermix, and 50 ng of DNA.The following cycling conditions were used: 5 min at 95 8C, followed by 40 cycles at 95 8C for 1 min, 60 8C for 1 min, and 72 8C for 1 min, followed by 7 min at 72 8C.Each individual from the HapMap was genotyped in replicate with the Deletion and Insertion1 primers while each individual in the HGDP was genotyped one time.In addition, each of the samples, hich appeared to be homozygous for the deletion, were genotyped using a second set of oligonucleotides for the insertion (Insertion2)

Human DNA samples.We genotyped 1,277 DNA samples corresponding to 270 samples from the International Hap Map project and 1,007 individuals from the CEPH HDGP [39,40].Our analysis from the HGDP includes individuals from 51 different populations and excludes samples previously identified as duplicates [21,41,42].Eight individuals (numbers 993, 994, 1028, 1030, 1031, 1033, 1034, and 1035) belonging to South African Bantu populations were genotyped (each was homozygous for the insertion) but were not included in the analysis due to small sample size.Individual genotypes are provided in Tables S1 and S4.

Haplotype construction.Phased SNP genotypes from HapMap Phase I were used for LD and extended haplotype analyses (http:// hapmap.org).We excluded SNPs mapping within the deleted region and used PHASE version 2.1 to infer new haplotypes which included the insertion/deletion genotypes (http://www.stat.washington.edu/stephens/software.html)[43,44].We also constructed haplotypes for insertion/deletion alleles based on the more complete Phase II genotyping data using only SNPs genotyped in all populations (Table S6).

Population genetic analysis.We used an exact test of Hardy-Weinberg equilibrium for two-allele loci as implemented in version 1.2.0 of the R genetics package [45].LD was measured by r 2 .F ST values were calculated from population allele frequencies using an unbiased estimator [46,47].The calculated F ST values were compared with an empirical distribution defined by a collection of SNPs and small indels genotyped in the same individuals [21,22].

EHH, REHH, and EHL were calculated for the locus using SWEEP (http://www.broad.mit.edu/mpg/sweep/index.html) version 1.0 [24].EHH is the probability that two randomly chosen chromosomes carrying the same allele at a core region are homozygous for all SNPs to a defined distance (x) from the core.REHH measures the decay of EHH at a given core genotype compared to the decay of other core haplotypes.SNPs mapping within the deleted region (five SNPs in CEU and two SNPs each in JPT and CHB and YRI) were e cluded for all analyses of both the insertion and deletion cores.We measured REHH values at a marker H of 0.04, which is a measure of the observed amount of recombination and is roughly equal to a genetic distance of 0.25 cM.Observed REHH values on each side of the core were compared with REHH values calculated for each HapMap Phase I SNP.EHL is operationally defined as the sum of the genetic distance at which EHH falls to 0.5 on either side of the core [48].This distance is sensitive to the density of markers, so SNP density was controlled by matching to the density around APOBEC3 using SWEEP (approximately one SNP every 2,500 bases).In this analysis, the core haplotype was defined as simply the insertion or deletion genotype.Comparisons were made with an empirical distribution calculated from HapMap Phase I data using two different definitions for the core region.First, we defined the core as the longest non-overlapping haplotypes containing between three and ten SNPs [49].Secondly, we treated each SNP locus individually as

e then divided int
20 bins based on the core frequency (intervals of 5%), and the values corresponding to the insertion and deletion alleles for each population were compared.

For the EHL analysis, haplotype length w