Associations of Filaggrin Gene Loss-of-Function Variants with Urinary Phthalate Metabolites and Testicular Function in Young Danish Men

http://dx.doi.org/10.1289/ehp.1306720



Table 2. Basic characteristics, unadjusted serum concentrations of reproductive hormones, and semen quality parameters by FLG genotype in 861 men.
Variable Missing (n) Unadjusted median (5th, 95th) Regression coefficient (95% CI) p-Valuea
FLG-null FLG WT
Abbreviations: cFT, calculated free testosterone; NA, not applicable. 5th and 95th are percentiles.
ap-Values represent the difference between genotype groups tested by multivariate linear regression. bBy Mann-Whitney test. cDependent variables were ln transformed. dAdjusted for BMI, smoking (number of cigarettes per day), alcohol consumption (units consumed in the week before participation), time of blood sampling, and age; also adjusted for type of assay in the inhibin-B model. eAdjusted for ejaculation abstinence time. fDependent variables were cubic root transformed. gDependent variable was square root transformed. hUnadjusted. iDependent variable was squared. jAdjusted for time from ejaculation to semen analysis.
Age (years) 4 19 (19, 21) 19 (18, 22) NA 0.1b
BMI (kg/m2) 7 21.9 (19, 28) 22.6 (19, 29) NA 0.04b
Alcohol use (units/week) 34 14 (0, 49) 12 (0, 42) NA 0.9b
Smoking (no. of cigarettes/day) 13 0 (0, 15) 0 (0, 20) NA 0.5b
Urinary osmolality (Osm/kg) 0 0.9 (0.4, 1.1) 0.9 (0.3, 1.1) NA 0.07b
Testosterone (nmol/L) 0 19 (12, 31) 19 (11, 30) –0.01 (–0.08, 0.07)c 0.9d
Estradiol (nmol/L) 0 75 (48, 127) 77 (45, 126) 0.01 (–0.07, 0.09)c 0.7d
SHBG (nmol/L) 0 29 (18, 50) 27 (12, 51) –0.09 (–0.19, 0.01)c 0.07d
LH (IU/L) 0 3.1 (1.4, 6.4) 3.0 (1.4, 6.2) –0.07 (–0.18, 0.04)c 0.2d
Inhibin-B (pg/mL) 0 188 (81, 298) 175 (98, 304) –0.03 (–0.13, 0.07)c 0.6d
FSH (IU/L) 0 2.7 (1.2, 5.8) 2.3 (0.9, 5.6) –0.14 (–0.28, 0.01)c 0.07d
cFT (ng/dL) 0 12 (7.9, 20) 12 (7.6, 21) 0.02 (–0.07, 0.10)c 0.7d
cFT/LH ratio 0 3.7 (1.9, 7.8) 4.1 (2.0, 8.6) 0.08 (–0.03, 0.20)c 0.2d
Testosterone/estradiol ratio 0 262 (150, 389) 242 (149, 382) –0.02 (–0.09, 0.05)c 0.6d
Testosterone/LH ratio 0 5.5 (2.9, 12) 6.2 (3.0, 12) 0.06 (–0.04, 0.17)c 0.2d
Inhibin-B/FSH ratio 0 70 (12, 197) 79 (19, 303) 0.11 (–0.11, 0.33)c 0.3d
Semen volume (mL) 6 3.1 (1.3, 5.8) 3.2 (1.3, 6.3) 1.29 (1.16, 1.42)c 0.8e
Sperm concentration (106/mL) 3 48 (1.9, 172) 48 (4.4, 167) 3.95 (3.65, 4.26)f 0.8e
Total sperm count (106) 7 164 (5.6, 447) 142 (13, 537) 6.11 (5.66, 6.55)f 0.6e
Total normal sperm (106) 19 11 (0.5, 58) 10 (0.1, 55) 2.6 (2.33, 2.87)f 0.4e
Morphologically normal sperm (%) 19 7.0 (1.0, 17.0) 6.8 (0.5, 17.0) 2.64 (2.39, 2.89)g 0.5h
Progressively motile sperm (%) 10 64 (28, 81) 61 (28, 80) 3,942 (3,524, 4,361)i 0.4j